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Antibody staining overlaps with antibody HPA054617.
Validated
Antibody staining overlaps with antibody HPA052612.
Standard validation
Approved
Approved
Figure description
Immunofluorescent staining of human cell line U-251 MG shows localization to centrosome.
Immunofluorescent staining of human cell line U-251 MG shows localization to centrosome.
Antibody dilution
1:122
1:44
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is partly supported by literature or no literature is available.
WESTERN BLOT
Antibody HPA052612
Antibody HPA054617
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
172.7, 172.5, 169.2, 163.8, 163.4, 159.9
172.7, 172.5, 169.2, 163.8, 163.4, 159.9, 92
Antibody dilution
1:610
1:220
PROTEIN ARRAY
Antibody HPA052612
Antibody HPA054617
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
