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Antibody staining overlaps with antibody HPA056813.
Validated
Antibody staining overlaps with antibody HPA051280.
Standard validation
Supported
Supported
Supported
Figure description
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
Antibody dilution
1:88
1:122
1:150
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA051280
Antibody HPA056813
Antibody CAB005355
Formal validation: Independent
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Pearson correlation with HPA056813 across 46 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA051280 across 46 cell lines.
Standard validation
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in both neuronal cells and glial cells.
Immunohistochemical staining of human cerebellum shows strong nuclear positivity in Purkinje cells, cells in molecular layer and cells in granular layer.
Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity.
Expression
RNA: detected in 37 tissues Protein: detected in 75 cell types
RNA: detected in 37 tissues Protein: detected in 78 cell types
RNA: detected in 37 tissues Protein: detected in 79 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:1000
1:300
1:600
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA051280
Antibody HPA056813
Antibody CAB005355
Formal validation: Genetic
Validated
Downregulation visible in one of two siRNA lanes
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: siRNA 1 Lane 3: siRNA 2 Lane 4: Scrambled
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
48.2, 44
48.2, 44
48.2, 44
Antibody dilution
1:440
1:410
1:500
PROTEIN ARRAY
Antibody HPA051280
Antibody HPA056813
Antibody CAB005355
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
