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Antibody staining overlaps with GFP tagged protein
Validated
Antibody staining overlaps with GFP tagged protein
Figure description
Immunofluorescent staining of transgenic HeLa cells show antibody staining in nuclear bodies & nucleoplasm and GFP expression in nucleoplasm.
Immunofluorescent staining of transgenic HeLa cells show antibody staining in nucleoplasm and GFP expression in nucleoplasm.
Antibody dilution
1:169
1:161
Formal validation: Independent
Validated
Antibody staining overlaps with antibody HPA069375. Antibody staining overlaps with antibody HPA068117.
Validated
Antibody staining overlaps with antibody HPA069375. Antibody staining overlaps with antibody HPA012035.
Validated
Antibody staining overlaps with antibody HPA068117. Antibody staining overlaps with antibody HPA012035.
Standard validation
Supported
Supported
Supported
Supported
Figure description
Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear bodies.
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
Antibody dilution
1:164
1:169
1:161
1:22
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
The subcellular location is supported by literature.
The subcellular location is supported by literature.
WESTERN BLOT
Antibody HPA012035
Antibody HPA057303
Antibody HPA068117
Antibody HPA069375
Standard validation
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
98.8, 76.5, 54.1, 52.8
98.8
98.8, 76.5, 54.1, 52.8, 16.2
98.8
Antibody dilution
1:250
1:560
1:530
1:80
PROTEIN ARRAY
Antibody HPA012035
Antibody HPA057303
Antibody HPA068117
Antibody HPA069375
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
