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Immunofluorescent staining of human cell line SH-SY5Y shows localization to the Golgi apparatus.
Antibody dilution
1:5
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA000339
Antibody CAB025136
Antibody CAB025269
Standard validation
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human kidney shows distinct membranous positivity in tubular cells.
Immunohistochemical staining of human small intestine shows strong positivity in luminal membrane of glandular cells.
Immunohistochemical staining of human small intestine shows strong luminal membranous positivity in glandular cells.
Expression
RNA: detected in 19 tissues Protein: detected in 15 cell types
RNA: detected in 19 tissues Protein: detected in 5 cell types
RNA: detected in 19 tissues Protein: detected in 5 cell types
Retrieval
HIER pH9
HIER pH6
HIER pH6
Antibody dilution
1:25
1:20000
1:1500
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Mainly consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA000339
Antibody CAB025136
Antibody CAB025269
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
75.6
75.6, 20.4
75.6, 20.4
Antibody dilution
1:250
1:500
1:500
PROTEIN ARRAY
Antibody HPA000339
Antibody CAB025136
Antibody CAB025269
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
