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Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Pearson correlation with HPA056798 across 44 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA044572 across 44 cell lines.
Standard validation
Supported
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human epididymis shows distinct positivity in peripheral nerves.
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells. Distinct staining was observed in fraction of neuropil.
Immunohistochemical staining of human epididymis shows distinct positivity in peripheral nerves.
Immunohistochemical staining of human epididymis shows distinct positivity in peripheral nerves.
Expression
RNA: detected in 17 tissues Protein: detected in 30 cell types
RNA: detected in 17 tissues Protein: detected in 10 cell types
RNA: detected in 17 tissues Protein: detected in 57 cell types
RNA: detected in 17 tissues Protein: detected in 13 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:225
1:50
1:35
1:35
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Consistent with RNA expression data.
Standard validation
Supported
Figure description
Subsets of neurons are positive, as well as axonal processes observed in selected regions of the brain, most abundant in hypothalamus.
WESTERN BLOT
Antibody HPA009978
Antibody HPA044572
Antibody HPA056798
Antibody CAB016733
Antibody CAB034368
Standard validation
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
10.9
10.9
10.9
10.9
10.9
Antibody dilution
1:250
1:250
1:170
1:500
1:500
PROTEIN ARRAY
Antibody HPA009978
Antibody HPA044572
Antibody HPA056798
Antibody CAB016733
Antibody CAB034368
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
