We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Antibody staining overlaps with antibody HPA022264.
Validated
Antibody staining overlaps with antibody HPA005757.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm & cytosol.
Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm, nuclear bodies & cytosol.
Antibody dilution
1:35
1:200
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA005757
Antibody HPA022264
Antibody CAB033926
Standard validation
Approved
Approved
Approved
Figure description
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human small intestine shows distinct cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human testis shows strong nuclear positivity in cells of seminiferus ducts.
Expression
RNA: detected in 34 tissues Protein: detected in 72 cell types
RNA: detected in 34 tissues Protein: detected in 71 cell types
RNA: detected in 34 tissues Protein: detected in 67 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:60
1:120
1:100
Literature conformity
Not consistent with gene/protein characterization data.
Not consistent with gene/protein characterization data.
Not consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA005757
Antibody HPA022264
Antibody CAB033926
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY416223)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY416223)
Target mass (kDa)
31.3, 18.2
31.3, 25.5
31.3, 25.5, 18.2
Antibody dilution
1:250
1:250
1:500
PROTEIN ARRAY
Antibody HPA005757
Antibody HPA022264
Antibody CAB033926
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
