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ATF1

ANTIBODY INFORMATION
 
 

Antibody HPA055069

  

Antibody HPA055406

  

Antibody CAB016222

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA055069   HPA055406   sc-270  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 12   12   4  

References

      

Proper citation

 Atlas Antibodies Cat#HPA055069, RRID:AB_2682688   Atlas Antibodies Cat#HPA055406, RRID:AB_2682807   Santa Cruz Biotechnology Cat#sc-270, RRID:AB_2290030  

VALIDATION SUMMARY
 
 

Antibody HPA055069

  

Antibody HPA055406

  

Antibody CAB016222

  
Immunocytochemistry


Validated

Validated
  

Validated
   Supported  
Immunohistochemistry

Supported     Supported  
Western blot


Validated
   Uncertain   Uncertain  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA055069

  

Antibody HPA055406

  

Antibody CAB016222

  

Formal validation: Genetic
Validated

Significant downregulation > 25% by both siRNA:s.		      

     

Figure description

 Nuclear region of segmented cells in 10x-images      

Antibody dilution

 1:36      
 

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA055406.		  
Validated

Antibody staining overlaps with antibody HPA055069.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm.   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.   Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.  

Antibody dilution

 1:36   1:93   1:25  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA055069

  

Antibody HPA055406

  

Antibody CAB016222

  

Standard validation

 Supported     Supported  

     

Figure description

 Immunohistochemical staining of human kidney shows moderate nuclear positivity in renal tubules and cells in glomeruli.     Immunohistochemical staining of human kidney shows strong nuclear positivity.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 49 cell types		     RNA: detected in 37 tissues
Protein: detected in 73 cell types		  

Retrieval

 HIER pH6     HIER pH6  

Antibody dilution

 1:350     1:100  

Literature conformity

 Consistent with extensive gene/protein characterization data.     Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.     Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA055069

  

Antibody HPA055406

  

Antibody CAB016222

  

Formal validation: Genetic
Validated

Downregulation visible in both siRNA lanes		      

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		      

Target mass (kDa)

 29.2, 20.8, 17.7      

Loading control

 Total protein image      

Antibody dilution

 1:80      
 

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 29.2, 20.8, 17.7   29.2   29.2, 20.8, 17.7  

Antibody dilution

 1:180   1:460   1:500  

PROTEIN ARRAY
 
 

Antibody HPA055069

  

Antibody HPA055406

  

Antibody CAB016222

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:1450   1:3700    

ANTIGEN INFORMATION
 
 

Antibody HPA055069

  

Antibody HPA055406

  

Antibody CAB016222

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Not known  

Length (aa)

 64   34    

Antigen sequence

 
MEDSHKSTTSETAPQPGSAVQGAHISHIAQQVSSLSESEESQDSSDSIGS
SQKAHGILARRPSY
  
TYQIRTTPSATSLPQTVVMTSPVTLTSQTTKTDD
  

											 
																				

																		

										
											
Matching transcripts

												
																					ATF1-001 - ENSP00000262053 [100%] 
ATF1-003 - ENSP00000448592 [100%] 
ATF1-004 - ENSP00000448921 [100%] 

											 
																						ATF1-001 - ENSP00000262053 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
ATF1-001
ATF1-003
ATF1-004
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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