CDK2 - Antibodies - The Human Protein Atlas

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CDK2

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA066915

Antibody CAB013115

Provider

Atlas Antibodies
Sigma-Aldrich

BioSource

Product name

HPA066915

AHZ0142

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Not known

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA066915, RRID:AB_2685748

VALIDATION SUMMARY

Antibody HPA066915

Antibody CAB013115

Immunocytochemistry

Immunohistochemistry

Western blot

Uncertain

Protein array

Supported

IMMUNOCYTOCHEMISTRY

Antibody HPA066915

Antibody CAB013115

Formal validation: Tagged protein

Validated

Antibody staining overlaps with GFP tagged protein

Figure description

Immunofluorescent staining of transgenic HeLa cells show antibody staining in cytoplasm & nucleus but excluded from the nucleoli and GFP expression in cytoplasm & nucleus but excluded from the nucleoli.

Antibody dilution

1:164

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm & centrosome.

Antibody dilution

1:164

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA066915

Antibody CAB013115

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.

Standard validation

Supported

Figure description

Immunohistochemical staining of human duodenum shows nuclear and cytoplasmic positivity in glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 34 cell types

Retrieval

HIER pH6

Antibody dilution

1:50

Literature conformity

Partly consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA066915

Antibody CAB013115

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.