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ARHGAP40

ANTIBODY INFORMATION
 
 

Antibody HPA042636

  

Antibody HPA042840

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA042636   HPA042840  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 9   9  

References

    

Proper citation

 Atlas Antibodies Cat#HPA042636, RRID:AB_10962149   Atlas Antibodies Cat#HPA042840, RRID:AB_10963929  

VALIDATION SUMMARY
 
 

Antibody HPA042636

  

Antibody HPA042840

  
Immunocytochemistry

Approved   Approved  
Immunohistochemistry

Uncertain   Uncertain  
Western blot

Uncertain   Uncertain  
Protein array

Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA042636

  

Antibody HPA042840

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane, cytosol & centrosome.   Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.  

Antibody dilution

 1:104   1:200  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA042636

  

Antibody HPA042840

  

Standard validation

 Uncertain   Uncertain  

   

Figure description

 Immunohistochemical staining of human kidney shows moderate nuclear and cytoplasmic positivity in renal tubules.   Immunohistochemical staining of human stomach shows strong cytoplasmic and membranous positivity in glandular cells.  

Expression

 RNA: detected in 12 tissues
Protein: detected in 66 cell types		   RNA: detected in 12 tissues
Protein: detected in 66 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:1100   1:300  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Not consistent with RNA expression data.   Not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA042636

  

Antibody HPA042840

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

    

Target mass (kDa)

 68.9, 65   68.9, 65  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA042636

  

Antibody HPA042840

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:30000  

ANTIGEN INFORMATION
 
 

Antibody HPA042636

  

Antibody HPA042840

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 79   98  

Antigen sequence

 
PNLFLHQGRPPKLPKGKEKQLAEGAAEVVQIMVHYQDLLWTVASFLVAQV
RKLNDSSSRRPQLCDAGLKTWLRRMHADR
  
AGKWKAAETRLFGVPLDSLLEADHKVLPSTQVPLVLQALLSCLEKRGLDM
EGILRVPGSQARVKGLEQKLERDFYAGLFSWDEVHHNDASDLLKRFIR
 

Matching transcripts

 ARHGAP40-001 - ENSP00000243967 [100%]
ARHGAP40-201 - ENSP00000362442 [100%]
  ARHGAP40-001 - ENSP00000243967 [100%]
ARHGAP40-201 - ENSP00000362442 [100%]
 

Other gene match

    
ANTIGEN VIEW
ARHGAP40-001
ARHGAP40-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.