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Antibody staining overlaps with antibody HPA049907. Antibody staining overlaps with antibody HPA035060.
Validated
Antibody staining overlaps with antibody HPA049907. Antibody staining overlaps with antibody HPA035059.
Validated
Antibody staining overlaps with antibody HPA035060. Antibody staining overlaps with antibody HPA035059.
Standard validation
Supported
Supported
Supported
Figure description
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli.
Immunofluorescent staining of human cell line A-431 shows localization to nucleoli.
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli.
Antibody dilution
1:169
1:67
1:91
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA035059
Antibody HPA035060
Antibody HPA049907
Formal validation: Independent
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Pearson correlation with HPA035060 across 46 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA035059 across 46 cell lines.
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human cerebral cortex shows strong nucleolar positivity in neuronal cells.
Immunohistochemical staining of human cerebral cortex shows strong nucleolar positivity in neuronal cells.
Expression
RNA: detected in 37 tissues Protein: detected in 37 cell types
RNA: detected in 37 tissues Protein: detected in 56 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:300
1:300
Literature conformity
Partly consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA035059
Antibody HPA035060
Antibody HPA049907
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
78.9
78.9, 53.6
78.9, 53.6
Antibody dilution
1:250
1:250
1:460
PROTEIN ARRAY
Antibody HPA035059
Antibody HPA035060
Antibody HPA049907
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
