RUNX2 - Antibodies - The Human Protein Atlas

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RUNX2

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA022040

Antibody CAB008374

Antibody CAB062561

Antibody CAB068226

Provider

Atlas Antibodies
Sigma-Aldrich

MBL

Atlas Antibodies
Sigma-Aldrich

Product name

HPA022040

D130-3

AMAb90594

AMAb90591

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Not known

Protein A/G

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA022040, RRID:AB_1846086

MBL International Cat#D130-3, RRID:AB_590789

Atlas Antibodies Cat#AMAb90594, RRID:AB_2665599

Atlas Antibodies Cat#AMAb90591, RRID:AB_2665598

VALIDATION SUMMARY

Antibody HPA022040

Antibody CAB008374

Antibody CAB062561

Antibody CAB068226

Immunocytochemistry

Supported

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA022040

Antibody CAB008374

Antibody CAB062561

Antibody CAB068226

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.

Antibody dilution

1:100

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA022040

Antibody CAB008374

Antibody CAB062561

Antibody CAB068226

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.

Standard validation

Supported

Figure description

Immunohistochemical staining of human lymph node shows strong nuclear positivity in subsets of cells.

Immunohistochemical staining of human lymph node shows strong nuclear positivity in lymphoid cells outside reaction centra.

Immunohistochemical staining of human tonsil shows distinct positivity in subsets of non germinal center cells.

Immunohistochemical staining of human tonsil shows strong nuclear positivity in few germinal center cells and several non-germinal center cells.

Expression

RNA: detected in 29 tissues
Protein: detected in 10 cell types

RNA: detected in 29 tissues
Protein: detected in 26 cell types

RNA: detected in 29 tissues
Protein: detected in 19 cell types

RNA: detected in 29 tissues
Protein: detected in 17 cell types

Retrieval

HIER pH6

Antibody dilution

1:250

1:200

1:500

1:1000

Literature conformity

Partly consistent with extensive gene/protein characterization data.

RNA consistency

Consistent with RNA expression data.

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA022040

Antibody CAB008374

Antibody CAB062561

Antibody CAB068226

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

56.6, 55.1, 54.2, 52.7

57.7, 56.6, 55.1, 54.2, 52.7

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA022040

Antibody CAB008374

Antibody CAB062561

Antibody CAB068226

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA022040

Antibody CAB008374

Antibody CAB062561

Antibody CAB068226

Antigen

Recombinant protein fragment

Recombinant protein

Length (aa)

105

Antigen sequence

LNSAPSPFNPQGQSQITDPRQAQSSPPWSYDQSYPSYLSQMTSPSIHSTT
PLSSTRGTGLPAITDVPRRISGASELGPFSDPRQFPSISSLTESRFSNPR
MHYPA

Matching transcripts

RUNX2-004 - ENSP00000360491 [100%]
RUNX2-010 - ENSP00000485863 [100%]
RUNX2-201 - ENSP00000360486 [100%]
RUNX2-001 - ENSP00000352514 [83%]
RUNX2-002 - ENSP00000420707 [83%]
RUNX2-003 - ENSP00000360493 [83%]

Other gene match

ANTIGEN VIEW

RUNX2-001	RUNX2-002	RUNX2-003	RUNX2-004	RUNX2-010	RUNX2-201

RELEVANT PUBLICATIONS

Antibody HPA022040

Antibody CAB008374

Antibody CAB062561

Antibody CAB068226

From shape to cells: mouse models reveal mechanisms altering palate development in Apert syndrome
Martínez-Abadías N et al
Dis Model Mech 2013;6(3):768-79

RUNX2 correlates with subtype-specific breast cancer in a human tissue microarray, and ectopic expression of Runx2 perturbs differentiation in the mouse mammary gland
McDonald L et al
Dis Model Mech 2014;7(5):525-34

Runx2 contributes to the regenerative potential of the mammary epithelium
Ferrari N et al
Sci Rep 2015;5:15658

BCL11B expression in intramembranous osteogenesis during murine craniofacial suture development
Holmes G et al
Gene Expr Patterns 2015;17(1):16-25

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.