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ANTIBODY INFORMATION VALIDATION SUMMARY IMMUNOCYTOCHEMISTRY IMMUNOHISTOCHEMISTRY WESTERN BLOT PROTEIN ARRAY ANTIGEN INFORMATION RELEVANT PUBLICATIONS

ANTIBODY INFORMATION

 
 

Antibody HPA037528

 

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA037528  

Host species

Rabbit  

Clonality

pAb  

Purity

Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

 

Released in version

7  

References

 

Proper citation

 

VALIDATION SUMMARY

 
 

Antibody HPA037528

 
Immunocytochemistry

Supported  
Western blot

Uncertain  
Protein array

Supported  

IMMUNOCYTOCHEMISTRY

 
 

Antibody HPA037528

 

Standard validation

Supported  

 

Figure description

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli & cytosol.  

Antibody dilution

1:143  

Literature conformity

The subcellular location is supported by literature.  

WESTERN BLOT

 
 

Antibody HPA037528

 

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.
 

 

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
 

Target mass (kDa)

132.2, 128.2, 122, 104.5, 34.4  

Antibody dilution

1:250  

PROTEIN ARRAY

 
 

Antibody HPA037528

 

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.
 

 

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

1:3000  

ANTIGEN INFORMATION

 
 

Antibody HPA037528

 

Antigen

Recombinant protein fragment  

Length (aa)

82  

Antigen sequence

LDGVMVGWVDKDLAPGIADSLRHFKVLREKRIPPWMEVVLIPMTGKPSLY
PGLFLFTTPCRLVRPVQNLALGKEELIGTMEQ
 

Matching transcripts

POLR1B-001 - ENSP00000263331 [100%]
POLR1B-005 - ENSP00000405358 [100%]
POLR1B-015 - ENSP00000394408 [100%]
POLR1B-201 - ENSP00000437914 [100%]
POLR1B-202 - ENSP00000444136 [100%]
 

Other gene match

 
ANTIGEN VIEW
POLR1B-001
POLR1B-005
POLR1B-015
POLR1B-201
POLR1B-202
 

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