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Antibody staining overlaps with antibody HPA067232.
Validated
Antibody staining overlaps with antibody HPA060601.
Standard validation
Approved
Approved
Figure description
Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
Antibody dilution
1:8
1:92
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is partly supported by literature or no literature is available.
WESTERN BLOT
Antibody HPA060601
Antibody HPA067232
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
41.6
41.6
Antibody dilution
1:80
1:310
PROTEIN ARRAY
Antibody HPA060601
Antibody HPA067232
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:500
1:3650
ANTIGEN INFORMATION
Antibody HPA060601
Antibody HPA067232
Antigen
Recombinant protein fragment
Recombinant protein fragment
Length (aa)
24
51
Antigen sequence
PESCQQLAAGGHSRLIVLHYNHSG
RSREVCRAVLSFLCCGCLRLGMRGPGDCLARAVEAHSGASTTDSSLRPRD
S
