MAX - Antibodies - The Human Protein Atlas

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MAX

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA003474

Antibody CAB000328

Provider

Atlas Antibodies
Sigma-Aldrich

Santa Cruz Biotechnology

Product name

HPA003474

sc-197

Host species

Rabbit

Clonality

pAb

msAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Affinity

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA003474, RRID:AB_1079317

Santa Cruz Biotechnology Cat#sc-197, RRID:AB_2281783

VALIDATION SUMMARY

Antibody HPA003474

Antibody CAB000328

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA003474

Antibody CAB000328

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & the Golgi apparatus.

Antibody dilution

1:200

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA003474

Antibody CAB000328

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.

Standard validation

Supported

Figure description

Immunohistochemical staining of human appendix shows nuclear positivity in lymphoid tissue.

Immunohistochemical staining of human rectum shows strong nuclear positivity in glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 23 cell types

RNA: detected in 37 tissues
Protein: detected in 71 cell types

Retrieval

HIER pH6

Antibody dilution

1:75

1:1000

Literature conformity

Partly consistent with extensive gene/protein characterization data.

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA003474

Antibody CAB000328

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

18.3, 17.2, 15.4, 12.1

18.3, 17.2, 15.4, 14.3, 14.1, 14, 12.1, 11.5, 11, 10.7, 9.6, 8.7, 5.2

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA003474

Antibody CAB000328

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:2000

ANTIGEN INFORMATION

Antibody HPA003474

Antibody CAB000328

Antigen

Recombinant protein fragment

Synthetic peptide

Length (aa)

119

Antigen sequence

MSDNDDIEVESDEEQPRFQSAADKRAHHNALERKRRDHIKDSFHSLRDSV
PSLQGEKASRAQILDKATEYIQYMRRKNHTHQQDIDDLKRQNALLEQQVR
ALEKARSSAQLQTNYPSSD

Matching transcripts

MAX-002 - ENSP00000351490 [100%]
MAX-001 - ENSP00000351175 [92%]
MAX-004 - ENSP00000284165 [82%]
MAX-006 - ENSP00000452378 [82%]
MAX-201 - ENSP00000480127 [82%]

Other gene match

ANTIGEN VIEW

MAX-001	MAX-002	MAX-004	MAX-006	MAX-201

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.