CCR7 - Antibodies - The Human Protein Atlas

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CCR7

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA031383

Antibody HPA074467

Antibody CAB003796

Provider

Atlas Antibodies
Sigma-Aldrich

Epitomics

Product name

HPA031383

HPA074467

1471-1

Host species

Rabbit

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Supernatant

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA031383, RRID:AB_2673854

Atlas Antibodies Cat#HPA074467, RRID:AB_2686693

VALIDATION SUMMARY

Antibody HPA031383

Antibody HPA074467

Antibody CAB003796

Immunocytochemistry

Immunohistochemistry

Western blot

Uncertain

Protein array

Supported

IMMUNOCYTOCHEMISTRY

Antibody HPA031383

Antibody HPA074467

Antibody CAB003796

Formal validation: Independent

Validated

Antibody staining overlaps with antibody HPA074467.

Validated

Antibody staining overlaps with antibody HPA031383.

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.

Immunofluorescent staining of human cell line A549 shows localization to mitochondria.

Antibody dilution

1:51

1:38

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA031383

Antibody HPA074467

Antibody CAB003796

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.

Standard validation

Supported

Figure description

Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in lymphoid cells outside reaction centra.

Expression

RNA: detected in 26 tissues
Protein: detected in 21 cell types

Retrieval

None

Antibody dilution

1:250

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Consistent with RNA expression data.

WESTERN BLOT

Antibody HPA031383

Antibody HPA074467

Antibody CAB003796

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

42.9, 42.2

42.9, 42.2, 22.6

Antibody dilution

1:250

1:130

1:500

PROTEIN ARRAY

Antibody HPA031383

Antibody HPA074467

Antibody CAB003796

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:1500

ANTIGEN INFORMATION

Antibody HPA031383

Antibody HPA074467

Antibody CAB003796

Antigen

Recombinant protein fragment

Synthetic peptide

Length (aa)

Antigen sequence

GVKFRNDLFKLFKDLGCLSQEQLRQWSSCRHIRRSSMSVEAETTTTFS

QDEVTDDYIGDNTTVDYTLFESLCSKKDVRNFKA

Matching transcripts

CCR7-001 - ENSP00000246657 [100%]
CCR7-002 - ENSP00000462631 [100%]

Other gene match

ANTIGEN VIEW

CCR7-001	CCR7-002

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.