ASL - Antibodies - The Human Protein Atlas

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ASL

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA016646

Antibody CAB003696

Provider

Atlas Antibodies
Sigma-Aldrich

Abnova

Product name

HPA016646

H00000435-M01

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Protein A/G

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA016646, RRID:AB_1845096

Abnova Corporation Cat#H00000435-M01, RRID:AB_425316

VALIDATION SUMMARY

Antibody HPA016646

Antibody CAB003696

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA016646

Antibody CAB003696

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.

Antibody dilution

1:125

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA016646

Antibody CAB003696

Standard validation

Supported

Figure description

Immunohistochemical staining of human liver shows moderate nuclear and cytoplasmic positivity in hepatocytes

Immunohistochemical staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes

Expression

RNA: detected in 37 tissues
Protein: detected in 12 cell types

RNA: detected in 37 tissues
Protein: detected in 17 cell types

Retrieval

HIER pH6

Antibody dilution

1:2500

1:500

Literature conformity

Consistent with gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA016646

Antibody CAB003696

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 17.5
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

51.7, 49.5, 48.7

51.7, 49.5, 48.7, 27.4

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA016646

Antibody CAB003696

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA016646

Antibody CAB003696

Antigen

Recombinant protein fragment

Recombinant protein

Length (aa)

Antigen sequence

MASESGKLWGGRFVGAVDPIMEKFNASIAYDRHLWEVDVQGSKAYSRGLE
KAGLLTKAEMDQILHGLDKVAEEWAQGTFKLNSNDED

Matching transcripts

ASL-001 - ENSP00000307188 [100%]
ASL-004 - ENSP00000370219 [100%]
ASL-005 - ENSP00000378741 [100%]
ASL-006 - ENSP00000378740 [100%]

Other gene match

ANTIGEN VIEW

ASL-001	ASL-004	ASL-005	ASL-006

RELEVANT PUBLICATIONS

Antibody HPA016646

Antibody CAB003696

Aberrant expression and distribution of enzymes of the urea cycle and other ammonia metabolizing pathways in dogs with congenital portosystemic shunts
van Straten G et al
PLoS One 2014;9(6):e100077
Application: IHC

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.