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PLEKHG3

ANTIBODY INFORMATION
 
 

Antibody HPA063455

  

Antibody HPA065323

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA063455   HPA065323  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 14   14  

References

    

Proper citation

 Atlas Antibodies Cat#HPA063455, RRID:AB_2685015   Atlas Antibodies Cat#HPA065323, RRID:AB_2685467  

VALIDATION SUMMARY
 
 

Antibody HPA063455

  

Antibody HPA065323

  
Immunocytochemistry

Approved   Approved  
Western blot

Uncertain   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA063455

  

Antibody HPA065323

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane & cytosol.   Immunofluorescent staining of human cell line HaCaT shows localization to nucleoplasm, plasma membrane, centrosome & cytokinetic bridge.  

Antibody dilution

 1:57   1:200  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

WESTERN BLOT
 
 

Antibody HPA063455

  

Antibody HPA065323

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 134.4, 128.2, 80.9, 77.8   134.4, 128.2, 80.9, 77.8  

Antibody dilution

 1:190   1:850  

PROTEIN ARRAY
 
 

Antibody HPA063455

  

Antibody HPA065323

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:2300   1:15300  

ANTIGEN INFORMATION
 
 

Antibody HPA063455

  

Antibody HPA065323

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 73   81  

Antigen sequence

 
DSLSCQLSPEVDISVGVATEDSPSVNGMEPPSPGCPVEPDRSSCKKKESA
LSTRDRLLLDKIKSYYENAEHHD
  
PDGGETLYVTADLTLEDNRRVIVMEKGPLPSPTAGLEESSGQGPSSPVAL
LGQVQDFQQSAECQPKEEGSRDPADPSQQGR
 

Matching transcripts

 PLEKHG3-005 - ENSP00000450945 [100%]
PLEKHG3-010 - ENSP00000247226 [100%]
PLEKHG3-012 - ENSP00000450973 [100%]
PLEKHG3-201 - ENSP00000378183 [100%]
  PLEKHG3-005 - ENSP00000450945 [100%]
PLEKHG3-010 - ENSP00000247226 [100%]
PLEKHG3-012 - ENSP00000450973 [100%]
PLEKHG3-201 - ENSP00000378183 [100%]
 

Other gene match

    
ANTIGEN VIEW
PLEKHG3-005
PLEKHG3-010
PLEKHG3-012
PLEKHG3-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.