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FGD3

ANTIBODY INFORMATION
 
 

Antibody HPA020963

  

Antibody HPA021018

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA020963   HPA021018  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 11   11  

References

    

Proper citation

 Atlas Antibodies Cat#HPA020963, RRID:AB_2670542   Atlas Antibodies Cat#HPA021018, RRID:AB_2670564  

VALIDATION SUMMARY
 
 

Antibody HPA020963

  

Antibody HPA021018

  
Immunocytochemistry

  Approved  
Immunohistochemistry

Approved   Approved  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA020963

  

Antibody HPA021018

  

Standard validation

   Approved  

   

Figure description

   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & cytosol.  

Antibody dilution

   1:200  

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA020963

  

Antibody HPA021018

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human tonsil shows strong positivity in a subset of leukocytes.   Immunohistochemical staining of human bone marrow shows strong positivity in a subset of hematopoietic cells.  

Expression

 RNA: detected in 32 tissues
Protein: detected in 58 cell types		   RNA: detected in 32 tissues
Protein: detected in 46 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:700   1:250  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA020963

  

Antibody HPA021018

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

    

Target mass (kDa)

 79.4, 79.3   79.4, 79.3  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA020963

  

Antibody HPA021018

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA020963

  

Antibody HPA021018

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 79   105  

Antigen sequence

 
PPGPIAALGMPDTGPGSSSLGKLQALPVGPRAHCGDPVSLAAAGDGSPDI
GPTGELSGSLKIPNRDSGIDSPSSSVAGE
  
EEEKKEWIQIIQATIEKHKQNSETFKAFGGAFSQDEDPSLSPDMPITSTS
PVEPVVTTEGSSGAAGLEPRKLSSKTRRDKEKQSCKSCGETFNSITKRRH
HCKLC
 

Matching transcripts

 FGD3-001 - ENSP00000364631 [100%]
FGD3-201 - ENSP00000336914 [100%]
FGD3-202 - ENSP00000413833 [100%]
  FGD3-001 - ENSP00000364631 [100%]
FGD3-201 - ENSP00000336914 [100%]
FGD3-202 - ENSP00000413833 [100%]
 

Other gene match

    
ANTIGEN VIEW
FGD3-001
FGD3-201
FGD3-202
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.