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Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & microtubules.
Immunofluorescent staining of human cell line U-2 OS shows localization to microtubules.
Antibody dilution
1:36
1:62
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA031868
Antibody CAB017717
Standard validation
Approved
Figure description
Immunohistochemical staining of human small intestine shows moderate cytoplasmic positivity in glandular cells and distinct staining of blood vessels and smooth muscle cells.
Expression
RNA: detected in 37 tissues Protein: detected in 75 cell types
Retrieval
HIER pH6
Antibody dilution
1:250
Literature conformity
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA031868
Antibody CAB017717
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
46.8, 44.7, 20
46.8, 44.7, 22, 20
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA031868
Antibody CAB017717
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.