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RFPL1

ANTIBODY INFORMATION
 
 

Antibody HPA048320

  

Antibody HPA072380

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA048320   HPA072380  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

 RFPL2 - ENSG00000128253 [100%]
RFPL3 - ENSG00000128276 [93%]
   RFPL2 - ENSG00000128253 [96%]
RFPL3 - ENSG00000128276 [100%]
  

Released in version

 16   16  

References

    

Proper citation

 Atlas Antibodies Cat#HPA048320, RRID:AB_2680356   Atlas Antibodies Cat#HPA072380, RRID:AB_2686515  

VALIDATION SUMMARY
 
 

Antibody HPA048320

  

Antibody HPA072380

  
Immunocytochemistry

Approved   Approved  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA048320

  

Antibody HPA072380

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunofluorescent staining of human cell line SiHa shows localization to nuclear speckles.   Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.  

Antibody dilution

 1:32   1:49  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

WESTERN BLOT
 
 

Antibody HPA048320

  

Antibody HPA072380

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

    

Target mass (kDa)

 42.1, 35.5, 35.4, 32.2   42.1, 35.5, 35.4, 32.2  

Antibody dilution

 1:160   1:110  

PROTEIN ARRAY
 
 

Antibody HPA048320

  

Antibody HPA072380

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:15000   1:1950  

ANTIGEN INFORMATION
 
 

Antibody HPA048320

  

Antibody HPA072380

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 30   26  

Antigen sequence

 
IQLTTELGFWTVSLRDGGRLSATTVPLTFL
  
QEASSCPVCSDYLEKPMSLECGCTVC
 

Matching transcripts

 RFPL1-001 - ENSP00000346342 [87%]
  RFPL1-001 - ENSP00000346342 [96%]
 

Other gene match

 RFPL2 - ENSG00000128253 [100%]
RFPL3 - ENSG00000128276 [93%]
   RFPL2 - ENSG00000128253 [96%]
RFPL3 - ENSG00000128276 [100%]
  
ANTIGEN VIEW
RFPL1-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.