DCTD - Antibodies - The Human Protein Atlas

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DCTD

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA035894

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA035894

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA035894, RRID:AB_10673488

VALIDATION SUMMARY

Antibody HPA035894

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA035894

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.

Antibody dilution

1:77

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA035894

Standard validation

Approved

Figure description

Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Expression

RNA: detected in 37 tissues
Protein: detected in 68 cell types

Retrieval

HIER pH6

Antibody dilution

1:100

Literature conformity

Consistent with gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA035894

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

21, 20, 19, 13.4, 11, 8.2

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA035894

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA035894

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

QPCGPNMSEVSCKKRDDYLEWPEYFMAVAFLSAQRSKDPNSQVGACIVNS
ENKIVGIGYNGMPNGCSDDVLPWRRTAENKLDTKYPYVCHA

Matching transcripts

DCTD-001 - ENSP00000349576 [100%]
DCTD-002 - ENSP00000398194 [93%]
DCTD-006 - ENSP00000422662 [93%]
DCTD-007 - ENSP00000423182 [93%]
DCTD-009 - ENSP00000424017 [93%]
DCTD-015 - ENSP00000424050 [93%]
DCTD-016 - ENSP00000423894 [93%]
DCTD-014 - ENSP00000421792 [80%]

Other gene match

ANTIGEN VIEW

DCTD-001	DCTD-002	DCTD-006	DCTD-007	DCTD-009	DCTD-014	DCTD-015	DCTD-016

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.