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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & nucleoli fibrillar center.
Antibody dilution
1:143
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA023924
Antibody HPA031526
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
Immunohistochemical staining of human cerebellum shows strong nuclear positivity in purkinje cells.
Expression
RNA: detected in 37 tissues Protein: detected in 69 cell types
RNA: detected in 37 tissues Protein: detected in 75 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:100
1:50
Literature conformity
Partly consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA023924
Antibody HPA031526
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY422337)
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
26.2, 24.8
26.2, 24.8, 19.7, 17.1, 12.4, 12.3
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA023924
Antibody HPA031526
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
