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Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in macrophages.
Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in a subset of reaction center cells.
Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in macrophages.
Immunohistochemical staining of human tonsil shows cytopalsmic positivity in macrophages.
Immunohistochemical staining of human tonsil shows cytoplasmic positivity in macrophages.
Expression
RNA: detected in 37 tissues Protein: detected in 12 cell types
RNA: detected in 37 tissues Protein: detected in 10 cell types
RNA: detected in 37 tissues Protein: detected in 9 cell types
RNA: detected in 37 tissues Protein: detected in 7 cell types
RNA: detected in 37 tissues Protein: detected in 8 cell types
Retrieval
HIER pH6
HIER pH9
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:12000
1:15000
1:600
1:1000
1:10000
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
Consistent with RNA expression data.
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA048982
Antibody CAB000051
Antibody CAB000066
Antibody CAB072861
Antibody CAB072862
Standard validation
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
37.4, 34.7
37.4, 34.7, 18.6, 13.2
37.4, 34.7, 18.6, 13.2
37.4, 34.7, 18.6, 13.2
37.4, 34.7, 18.6, 13.2
Antibody dilution
1:350
1:500
1:500
1:1000
1:500
PROTEIN ARRAY
Antibody HPA048982
Antibody CAB000051
Antibody CAB000066
Antibody CAB072861
Antibody CAB072862
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
