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FXR2

ANTIBODY INFORMATION
 
 

Antibody HPA022997

  

Antibody CAB011205

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   SIGMA
  

Product name

 HPA022997   F1554  

Host species

 Rabbit   Mouse  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

    

Released in version

 5   4  

References

    

Proper citation

 Atlas Antibodies Cat#HPA022997, RRID:AB_1849208   Sigma-Aldrich Cat#F1554, RRID:AB_476964  

VALIDATION SUMMARY
 
 

Antibody HPA022997

  

Antibody CAB011205

  
Immunocytochemistry

Supported    
Immunohistochemistry

Supported   Supported  
Western blot


Validated
   Uncertain  
Protein array

Approved    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA022997

  

Antibody CAB011205

  

Standard validation

 Supported    

   

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.    

Antibody dilution

 1:100    

Literature conformity

 The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA022997

  

Antibody CAB011205

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.   Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 79 cell types		   RNA: detected in 37 tissues
Protein: detected in 79 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:200   1:8000  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA022997

  

Antibody CAB011205

  

Formal validation: Genetic
Validated

Downregulation visible in both siRNA lanes		    

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		    

Target mass (kDa)

 74.2    

Loading control

 Total protein image    

Antibody dilution

 1:289    
 

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

    

Target mass (kDa)

 74.2   74.2, 13.7  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA022997

  

Antibody CAB011205

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA022997

  

Antibody CAB011205

  

Antigen

 Recombinant protein fragment   Synthetic peptide  

Length (aa)

 77    

Antigen sequence

 
ERLQIDEQLRQIGLGFRPPGSGRGSGGSDKAGYSTDESSSSSLHATRTYG
GSYGGRGRGRRTGGPAYGPSSDVSTAS
  

											 
																				

																		

										
											
Matching transcripts

												
																					FXR2-001 - ENSP00000250113 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
FXR2-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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