Fields »
ABOUT
HELP
BLOG
KRI1

ANTIBODY INFORMATION
 
 

Antibody HPA043110

  

Antibody HPA043574

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA043110   HPA043574  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 10   10  

References

    

Proper citation

 Atlas Antibodies Cat#HPA043110, RRID:AB_2678321   Atlas Antibodies Cat#HPA043574, RRID:AB_2678561  

VALIDATION SUMMARY
 
 

Antibody HPA043110

  

Antibody HPA043574

  
Immunocytochemistry


Validated

Validated
  

Validated

Validated
  
Immunohistochemistry

Approved   Approved  
Western blot


Validated
   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA043110

  

Antibody HPA043574

  

Formal validation: Genetic
Validated

Significant downregulation > 25% by both siRNA:s.		  
Validated

Significant downregulation > 25% by one siRNA.		  

 
 

Figure description

 Nuclear region of segmented cells in 10x-images   Nuclear region of segmented cells in 10x-images  

Antibody dilution

 1:66   1:84  
 

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA043574.		  
Validated

Antibody staining overlaps with antibody HPA043110.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line MCF7 shows localization to nucleoli.   Immunofluorescent staining of human cell line MCF7 shows localization to nucleoli.  

Antibody dilution

 1:66   1:84  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA043110

  

Antibody HPA043574

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human cerebellum shows strong nucleolar positivity in Purkinje cells.   Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic and nucleolar positivity in neuronal cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 64 cell types		   RNA: detected in 37 tissues
Protein: detected in 57 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:35   1:600  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA043110

  

Antibody HPA043574

  

Formal validation: Genetic
Validated

Downregulation visible in both siRNA lanes		    

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		    

Target mass (kDa)

 83.3    

Loading control

 Total protein image    

Antibody dilution

 1:146    
 

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

    

Target mass (kDa)

 83.3   83.3  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA043110

  

Antibody HPA043574

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA043110

  

Antibody HPA043574

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 103   109  

Antigen sequence

 
QVFKSLCREEAETPAEATGKPQRDEAGPQRQLPALDGSLMGPESPPAQEE
EAPVSPHKKPAPQKRRRAKKARLLGPTVMLGGCEFSRQRLQAFGLNPKRL
HFR
  
KNLKRKEILAKLEKLRKVTGNEMLGLEEGDLEDDFDPAQHDQLMQKCFGD
EYYGAVEEEKPQFEEEEGLEDDWNWDTWDGPEQEGDWSQQELHCEDPNFN
MDADYDPSQ
 

Matching transcripts

 KRI1-004 - ENSP00000320917 [100%]
  KRI1-004 - ENSP00000320917 [100%]
 

Other gene match

    
ANTIGEN VIEW
KRI1-004
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.