We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm & vesicles.
Antibody dilution
1:30
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA001140
Standard validation
Supported
Figure description
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 18 cell types
Retrieval
HIER pH6
Antibody dilution
1:250
Literature conformity
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA001140
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401024)
Target mass (kDa)
17.6, 13
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA001140
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
