Fields »
ABOUT
HELP
BLOG
ARHGEF6

ANTIBODY INFORMATION
 
 

Antibody HPA003578

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA003578  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 2  

References

 1  

Proper citation

 Atlas Antibodies Cat#HPA003578, RRID:AB_1078203  

VALIDATION SUMMARY
 
 

Antibody HPA003578

  
Immunocytochemistry

Approved  
Immunohistochemistry

Approved  
Western blot

Supported  
Protein array

Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA003578

  

Standard validation

 Approved  

 

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.  

Antibody dilution

 1:44  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA003578

  

Standard validation

 Approved  

 

Figure description

 Immunohistochemical staining of human tonsil shows cytoplasmic positivity in immune cells.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 30 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:200  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA003578

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

 

Figure description

 Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 87.5, 70.8  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA003578

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA003578

  

Antigen

 Recombinant protein fragment  

Length (aa)

 111  

Antigen sequence

 
VQYGACEEKEERYLMLFSNVLIMLSASPRMSGFIYQGKIPIAGTVVTRLD
EIEGNDCTFEITGNTVERIVVHCNNNQDFQEWLEQLNRLIRGPASCSSLS
KTSSSSCSAHS
 

Matching transcripts

 ARHGEF6-001 - ENSP00000250617 [100%]
ARHGEF6-002 - ENSP00000359656 [100%]
ARHGEF6-003 - ENSP00000359654 [100%]
 

Other gene match

  
ANTIGEN VIEW
ARHGEF6-001
ARHGEF6-002
ARHGEF6-003

RELEVANT PUBLICATIONS
 
 

Antibody HPA003578

  

1.

 αPIX Is a Trafficking Regulator that Balances Recycling and Degradation of the Epidermal Growth Factor Receptor
Kortüm F et al
PLoS One 2015;10(7):e0132737
  
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.