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Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Antibody dilution
1:130
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA002809
Standard validation
Approved
Figure description
Immunohistochemical staining of human hippocampus shows strong nucleolar and cytoplasmic positivity in neuronal cells.
Expression
RNA: detected in 34 tissues Protein: detected in 38 cell types
Retrieval
HIER pH6
Antibody dilution
1:250
Literature conformity
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA002809
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY418207)
Target mass (kDa)
28.8, 27.6, 25.6, 22.3, 21.6
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA002809
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
