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Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & cytokinetic bridge.
Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm.
Antibody dilution
1:38
1:9
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA051281
Antibody HPA064673
Standard validation
Approved
Figure description
Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.
Expression
RNA: detected in 37 tissues Protein: detected in 62 cell types
Retrieval
HIER pH6
Antibody dilution
1:120
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA051281
Antibody HPA064673
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
84.8
84.8, 64.4
Antibody dilution
1:80
1:80
PROTEIN ARRAY
Antibody HPA051281
Antibody HPA064673
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
