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LBP

ANTIBODY INFORMATION
 
 

Antibody HPA001508

  

Antibody CAB025905

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   R&D Systems
  

Product name

 HPA001508   AF870  

Host species

 Rabbit   Goat  

Clonality

 pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

    

Released in version

 2   5  

References

 1    

Proper citation

 Atlas Antibodies Cat#HPA001508, RRID:AB_1079241    

VALIDATION SUMMARY
 
 

Antibody HPA001508

  

Antibody CAB025905

  
Immunohistochemistry

Approved   Approved  
Western blot

Uncertain   Supported  
Protein array

Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA001508

  

Antibody CAB025905

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human small intestine shows positivity in plasma.   Immunohistochemical staining of human colon shows positivity in endothelial cells and plasma.  

Expression

 RNA: detected in 8 tissues
Protein: detected in 12 cell types		   RNA: detected in 8 tissues
Protein: detected in 43 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:150   1:150  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA001508

  

Antibody CAB025905

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 53.4   53.4  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA001508

  

Antibody CAB025905

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA001508

  

Antibody CAB025905

  

Antigen

 Recombinant protein fragment   Recombinant protein  

Length (aa)

 137    

Antigen sequence

 
EGYLNFSITDDMIPPDSNIRLTTKSFRPFVPRLARLYPNMNLELQGSVPS
APLLNFSPGNLSVDPYMEIDAFVLLPSSSKEPVFRLSVATNVSATLTFNT
SKITGFLKPGKVKVELKESKVGLFNAELLEALLNYYI
  

											 
																				

																		

										
											
Matching transcripts

												
																					LBP-001 - ENSP00000217407 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
LBP-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA001508

												
										 
																				
											
											
											
Antibody CAB025905

												
										 
																		

																	

										
											
1.

												
																						Affinity proteomics reveals elevated muscle proteins in plasma of children with cerebral malaria

	Bachmann J et al

	PLoS Pathog 2014;10(4):e1004038

			
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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