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Immunohistochemical staining of human cerebral cortex shows distinct positivity in neuropil.
Expression
RNA: detected in 16 tissues Protein: detected in 4 cell types
Retrieval
HIER pH6
Antibody dilution
1:450
Literature conformity
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Standard validation
Supported
Figure description
Subsets of neurons displayed positivity in their cell bodies and dendrites. Most abundant staining was observed in olfactory bulb, cerebral cortex and hippocampus among others.
WESTERN BLOT
Antibody HPA050509
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Figure description
Target mass (kDa)
97.6, 91.3, 91.2, 86.3
Antibody dilution
1:180
PROTEIN ARRAY
Antibody HPA050509
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
