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Immunofluorescent staining of human cell line MCF7 shows localization to nucleus, nucleoli & cytosol.
Antibody dilution
1:119
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA040930
Antibody HPA041730
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human colon shows moderate nuclear positivity in glandular cells.
Immunohistochemical staining of human colon shows moderate cytoplasmic and membranous positivity in glandular cells.
Expression
RNA: detected in 36 tissues Protein: detected in 60 cell types
RNA: detected in 36 tissues Protein: detected in 47 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:200
1:200
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA040930
Antibody HPA041730
Standard validation
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY406389)
Target mass (kDa)
37.3, 28.7
37.3
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA040930
Antibody HPA041730
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
