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Antibody staining overlaps with antibody HPA045590.
Validated
Antibody staining overlaps with antibody HPA020536.
Standard validation
Supported
Supported
Approved
Figure description
Immunofluorescent staining of human cell line A-431 shows localization to nucleus & nucleoli.
Immunofluorescent staining of human cell line MCF7 shows localization to nucleus & nucleoli.
Immunofluorescent staining of human cell line A-431 shows localization to nucleus, nucleoli & cytosol.
Antibody dilution
1:100
1:7
1:55
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA020536
Antibody HPA045590
Antibody HPA046867
Standard validation
Approved
Figure description
Immunohistochemical staining of human rectum shows strong nuclear and cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 53 cell types
Retrieval
HIER pH6
Antibody dilution
1:250
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA020536
Antibody HPA045590
Antibody HPA046867
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
51.1, 50.4, 46.7, 42.5
51.1, 50.4, 46.7, 42.5
50.4
Antibody dilution
1:250
1:250
1:270
PROTEIN ARRAY
Antibody HPA020536
Antibody HPA045590
Antibody HPA046867
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
