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MLLT4

ANTIBODY INFORMATION
 
 

Antibody HPA030212

  

Antibody HPA030213

  

Antibody HPA030214

  

Antibody HPA030215

  

Antibody HPA049868

  

Antibody CAB013496

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Zymed
  

Product name

 HPA030212   HPA030213   HPA030214   HPA030215   HPA049868   36-4300  

Host species

 Rabbit   Rabbit   Rabbit   Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb   pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

            

Released in version

 6   6   6   6   12   4  

References

            

Proper citation

 Atlas Antibodies Cat#HPA030212, RRID:AB_10599291   Atlas Antibodies Cat#HPA030213, RRID:AB_10601340   Atlas Antibodies Cat#HPA030214, RRID:AB_10601005   Atlas Antibodies Cat#HPA030215, RRID:AB_10601556   Atlas Antibodies Cat#HPA049868, RRID:AB_2680924    

VALIDATION SUMMARY
 
 

Antibody HPA030212

  

Antibody HPA030213

  

Antibody HPA030214

  

Antibody HPA030215

  

Antibody HPA049868

  

Antibody CAB013496

  
Immunocytochemistry


Validated
  

Validated
    

Validated
   Supported   Uncertain  
Immunohistochemistry

Approved   Approved   Approved   Approved     Approved  
Western blot

Supported   Uncertain   Uncertain   Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported   Supported   Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA030212

  

Antibody HPA030213

  

Antibody HPA030214

  

Antibody HPA030215

  

Antibody HPA049868

  

Antibody CAB013496

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA030215.
Antibody staining overlaps with antibody HPA030213.		  
Validated

Antibody staining overlaps with antibody HPA030212.		    
Validated

Antibody staining overlaps with antibody HPA030212.		      
 

Standard validation

 Supported   Supported     Supported   Supported   Uncertain  

           

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane & cell junctions.   Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles, plasma membrane & cell junctions.     Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & cell junctions.   Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cell junctions.   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & cell junctions.  

Antibody dilution

 1:400   1:200     1:50   1:83   1:44  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.     The subcellular location is supported by literature.   The subcellular location is supported by literature.   The subcellular location is not consistent with literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA030212

  

Antibody HPA030213

  

Antibody HPA030214

  

Antibody HPA030215

  

Antibody HPA049868

  

Antibody CAB013496

  

Standard validation

 Approved   Approved   Approved   Approved     Approved  

           

Figure description

 Immunohistochemical staining of human pancreas shows distinct positivity in acinar luminal membranes.   Immunohistochemical staining of human epididymis shows strong membranous positivity in glandular cells.   Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.   Immunohistochemical staining of human pancreas shows moderate granular positivity in exocrine cells.     Immunohistochemical staining of human pancreas shows distinct positivity in intercalated ducts.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 51 cell types		   RNA: detected in 37 tissues
Protein: detected in 70 cell types		   RNA: detected in 37 tissues
Protein: detected in 52 cell types		   RNA: detected in 37 tissues
Protein: detected in 56 cell types		     RNA: detected in 37 tissues
Protein: detected in 28 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6     HIER pH6  

Antibody dilution

 1:400   1:400   1:400   1:250     1:175  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.     Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.     Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA030212

  

Antibody HPA030213

  

Antibody HPA030214

  

Antibody HPA030215

  

Antibody HPA049868

  

Antibody CAB013496

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

           

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		            

Target mass (kDa)

 207.8, 207.6, 206.8, 201.8, 197.7, 189.2, 187.7, 43.2   207.8, 207.6, 206.8, 201.8, 197.7, 189.2, 187.7   207.8, 207.6, 206.8, 201.8, 197.7, 189.2, 187.7   207.8, 207.6, 206.8, 201.8, 197.7, 189.2, 187.7, 18.8   207.8, 207.6, 206.8, 201.8, 197.7, 189.2, 187.7, 29.1, 28.9   207.8, 207.6, 206.8, 201.8, 197.7, 189.2, 187.7, 43.2, 36.4, 29.1, 28.9, 20.6, 18.8, 16, 13.1  

Antibody dilution

 1:250   1:250   1:250   1:250   1:100   1:500  

PROTEIN ARRAY
 
 

Antibody HPA030212

  

Antibody HPA030213

  

Antibody HPA030214

  

Antibody HPA030215

  

Antibody HPA049868

  

Antibody CAB013496

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

           

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:3000   1:3000   1:3000   1:3350    

ANTIGEN INFORMATION
 
 

Antibody HPA030212

  

Antibody HPA030213

  

Antibody HPA030214

  

Antibody HPA030215

  

Antibody HPA049868

  

Antibody CAB013496

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Not known  

Length (aa)

 101   103   81   86   63    

Antigen sequence

 
ASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHS
GTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQ
D
  
KPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLS
PDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLML
EWQ
  
DSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKD
YCIARVMLPPGAQHSDEKGAKEIILDDDECP
  
EAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSL
QLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADE
  
DDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKR
MQEFRSSDGRPDS
  

											 
																				

																		

										
											
Matching transcripts

												
																					MLLT4-002 - ENSP00000341118 [100%] 
MLLT4-003 - ENSP00000383623 [100%] 
MLLT4-004 - ENSP00000252692 [100%] 
MLLT4-005 - ENSP00000414675 [100%] 
MLLT4-013 - ENSP00000404595 [100%] 
MLLT4-014 - ENSP00000375956 [100%] 
MLLT4-201 - ENSP00000355771 [100%] 
MLLT4-202 - ENSP00000375960 [100%] 

											 
																						MLLT4-002 - ENSP00000341118 [100%] 
MLLT4-003 - ENSP00000383623 [100%] 
MLLT4-004 - ENSP00000252692 [100%] 
MLLT4-013 - ENSP00000404595 [100%] 
MLLT4-014 - ENSP00000375956 [100%] 
MLLT4-201 - ENSP00000355771 [100%] 
MLLT4-202 - ENSP00000375960 [100%] 

											 
																						MLLT4-002 - ENSP00000341118 [100%] 
MLLT4-003 - ENSP00000383623 [100%] 
MLLT4-004 - ENSP00000252692 [100%] 
MLLT4-013 - ENSP00000404595 [100%] 
MLLT4-014 - ENSP00000375956 [100%] 
MLLT4-201 - ENSP00000355771 [100%] 
MLLT4-202 - ENSP00000375960 [100%] 

											 
																						MLLT4-002 - ENSP00000341118 [100%] 
MLLT4-003 - ENSP00000383623 [100%] 
MLLT4-004 - ENSP00000252692 [100%] 
MLLT4-013 - ENSP00000404595 [100%] 
MLLT4-014 - ENSP00000375956 [100%] 
MLLT4-201 - ENSP00000355771 [100%] 
MLLT4-202 - ENSP00000375960 [100%] 
MLLT4-009 - ENSP00000426462 [87%] 

											 
																						MLLT4-002 - ENSP00000341118 [100%] 
MLLT4-003 - ENSP00000383623 [100%] 
MLLT4-004 - ENSP00000252692 [100%] 
MLLT4-010 - ENSP00000383626 [100%] 
MLLT4-011 - ENSP00000383625 [100%] 
MLLT4-013 - ENSP00000404595 [100%] 
MLLT4-014 - ENSP00000375956 [100%] 
MLLT4-201 - ENSP00000355771 [100%] 
MLLT4-202 - ENSP00000375960 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

					«					
						

							

								

									
MLLT4-002
MLLT4-003
MLLT4-004
MLLT4-005
MLLT4-009
MLLT4-010
MLLT4-011
MLLT4-013
MLLT4-014
MLLT4-201
MLLT4-202
								

							

						

							
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