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TNNI2

ANTIBODY INFORMATION
 
 

Antibody HPA055938

  

Antibody CAB018697

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA055938   sc-48347  

Host species

 Rabbit   Mouse  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

    

Released in version

 13   4  

References

    

Proper citation

 Atlas Antibodies Cat#HPA055938, RRID:AB_2682976   Santa Cruz Biotechnology Cat#sc-48347, RRID:AB_628400  

VALIDATION SUMMARY
 
 

Antibody HPA055938

  

Antibody CAB018697

  
Immunocytochemistry

Approved    
Immunohistochemistry

Supported   Supported  
Western blot

Supported   Supported  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA055938

  

Antibody CAB018697

  

Standard validation

 Approved    

   

Figure description

 Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm & intermediate filaments.    

Antibody dilution

 1:200    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA055938

  

Antibody CAB018697

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.   Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.  

Expression

 RNA: detected in 21 tissues
Protein: detected in 20 cell types		   RNA: detected in 21 tissues
Protein: detected in 2 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:2800   1:2000  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA055938

  

Antibody CAB018697

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 21.4, 21.3   21.4, 21.3  

Antibody dilution

 1:1200   1:500  

PROTEIN ARRAY
 
 

Antibody HPA055938

  

Antibody CAB018697

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:9600    

ANTIGEN INFORMATION
 
 

Antibody HPA055938

  

Antibody CAB018697

  

Antigen

 Recombinant protein fragment   Not known  

Length (aa)

 63    

Antigen sequence

 
ELEKEESRREAEKQNYLAEHCPPLHIPGSMSEVQELCKQLHAKIDAAEEE
KYDMEVRVQKTSK
  

											 
																				

																		

										
											
Matching transcripts

												
																					TNNI2-001 - ENSP00000371331 [100%] 
TNNI2-002 - ENSP00000371336 [100%] 
TNNI2-003 - ENSP00000371330 [100%] 
TNNI2-201 - ENSP00000252898 [100%] 
TNNI2-202 - ENSP00000481242 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
TNNI2-001
TNNI2-002
TNNI2-003
TNNI2-201
TNNI2-202
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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