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Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
Antibody dilution
1:26
Literature conformity
The subcellular location is not consistent with literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA056833
Standard validation
Supported
Figure description
Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in Leydig cells.
Expression
RNA: detected in 17 tissues Protein: detected in 13 cell types
Retrieval
HIER pH6
Antibody dilution
1:80
Literature conformity
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA056833
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY424871)
Target mass (kDa)
34.5, 29.1
Antibody dilution
1:125
PROTEIN ARRAY
Antibody HPA056833
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:950
ANTIGEN INFORMATION
Antibody HPA056833
Antigen
Recombinant protein fragment
Length (aa)
51
Antigen sequence
NVGILPNLLPSHFLNAPDEIQSLIHCNITSVVKMTQLILKHMESRQKGLI
L
