Fields »
ABOUT
HELP
BLOG
AKAP12

ANTIBODY INFORMATION
 
 

Antibody HPA006344

  

Antibody HPA056230

  

Antibody CAB026379

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Origene
  

Product name

 HPA006344   HPA056230   3535.00.02  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

      

Released in version

 3   13   13  

References

 1      

Proper citation

 Atlas Antibodies Cat#HPA006344, RRID:AB_1078118   Atlas Antibodies Cat#HPA056230, RRID:AB_2683073    

VALIDATION SUMMARY
 
 

Antibody HPA006344

  

Antibody HPA056230

  

Antibody CAB026379

  
Immunocytochemistry


Validated
  

Validated
    
Immunohistochemistry


Validated

Validated
  

Validated

Validated
  

Validated
  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA006344

  

Antibody HPA056230

  

Antibody CAB026379

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA056230.		  
Validated

Antibody staining overlaps with antibody HPA006344.		    
 

Standard validation

 Supported   Supported    

     

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.   Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.    

Antibody dilution

 1:9   1:116    

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA006344

  

Antibody HPA056230

  

Antibody CAB026379

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		    

 
   

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.    
 

Formal validation: Orthogonal

    
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

   
 

Figure description

     Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.  
 

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		    

 
   

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA056230 across 46 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA006344 across 46 cell lines.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human testis shows strong cytoplasmic and membranous positivity in seminiferus ducts.   Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells n seminiferus ducts.   Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells of seminiferus ducts.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 33 cell types		   RNA: detected in 37 tissues
Protein: detected in 22 cell types		   RNA: detected in 37 tissues
Protein: detected in 14 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:75   1:1000   1:1000  

Literature conformity

 No avaliable gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA006344

  

Antibody HPA056230

  

Antibody CAB026379

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

     

Figure description

      

Target mass (kDa)

 191.5, 181.7, 181   191.5, 181.7, 181   191.5, 181.7, 181  

Antibody dilution

 1:250   1:580   1:500  

PROTEIN ARRAY
 
 

Antibody HPA006344

  

Antibody HPA056230

  

Antibody CAB026379

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500   1:4650    

ANTIGEN INFORMATION
 
 

Antibody HPA006344

  

Antibody HPA056230

  

Antibody CAB026379

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Genetic immunization  

Length (aa)

 106   147    

Antigen sequence

 
QRAEAERPEEQAEASGLKKETDVVLKVDAQEAKTEPFTQGKVVGQTTPES
FEKAPQVTESIESSELVTTCQAETLAGVKSQEMVMEQAIPPDSVETPTDS
ETDGST
  
SKDMSEASEKTMTVEVEGSTVNDQQLEEVVLPSEEEGGGAGTKSVPEDDG
HALLAERIEKSLVEPKEDEKGDDVDDPENQNSALADTDASGGLTKESPDT
NGPKQKEKEDAQEVELQEGKVHSESDKAITPQAQEELQKQERESAKS
  

											 
																				

																		

										
											
Matching transcripts

												
																					AKAP12-001 - ENSP00000253332 [100%] 
AKAP12-002 - ENSP00000346702 [100%] 
AKAP12-201 - ENSP00000352794 [100%] 
AKAP12-202 - ENSP00000384537 [100%] 

											 
																						AKAP12-001 - ENSP00000253332 [100%] 
AKAP12-002 - ENSP00000346702 [100%] 
AKAP12-201 - ENSP00000352794 [100%] 
AKAP12-202 - ENSP00000384537 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
AKAP12-001
AKAP12-002
AKAP12-201
AKAP12-202
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA006344

												
										 
																				
											
											
											
Antibody HPA056230

												
										 
																				
											
											
											
Antibody CAB026379

												
										 
																		

																	

										
											
1.

												
																						Elevated AKAP12 in paclitaxel-resistant serous ovarian cancer cells is prognostic and predictive of poor survival in patients

	Bateman NW et al

	J Proteome Res 2015;14(4):1900-10

			Application: WB				
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

					Contact
				

				
				

					
					
					
					
				

				
			


			

				

					The Project
				
			
				
			

			
			

			
				

					The Human Protein Atlas
				

				
			

			
			

			

						The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.