NFATC1 - Antibodies - The Human Protein Atlas

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NFATC1

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA071732

Antibody CAB004513

Provider

Atlas Antibodies
Sigma-Aldrich

Santa Cruz Biotechnology

Product name

HPA071732

sc-17834

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Protein A/G

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA071732, RRID:AB_2686439

Santa Cruz Biotechnology Cat#sc-17834, RRID:AB_628011

VALIDATION SUMMARY

Antibody HPA071732

Antibody CAB004513

Immunocytochemistry

Supported

Immunohistochemistry

Western blot

Supported

Uncertain

Protein array

Supported

IMMUNOCYTOCHEMISTRY

Antibody HPA071732

Antibody CAB004513

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & nuclear bodies.

Antibody dilution

1:85

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA071732

Antibody CAB004513

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.

Standard validation

Supported

Figure description

Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in reaction center cells.

Expression

RNA: detected in 36 tissues
Protein: detected in 24 cell types

Retrieval

HIER pH6

Antibody dilution

1:75

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA071732

Antibody CAB004513

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

101.2, 100.3, 88.8, 87.9, 77.8, 77.4, 76.9, 76.5, 69.4

101.2, 100.3, 88.8, 87.9, 77.8, 77.4, 76.9, 76.5, 69.4, 51.1, 38.6

Antibody dilution

1:280

1:500

PROTEIN ARRAY

Antibody HPA071732

Antibody CAB004513

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3400

ANTIGEN INFORMATION

Antibody HPA071732

Antibody CAB004513

Antigen

Recombinant protein fragment

Recombinant protein

Length (aa)

Antigen sequence

GYGAALDGGPAGYFLSSGHTRPDGAPALESPRIEITSCLGLYHNNNQFFH
DVEVEDVLPSSKRSPSTATLSLPSLEAYRD

Matching transcripts

NFATC1-001 - ENSP00000253506 [100%]
NFATC1-003 - ENSP00000316553 [100%]
NFATC1-004 - ENSP00000466194 [100%]
NFATC1-005 - ENSP00000468111 [100%]
NFATC1-006 - ENSP00000442435 [100%]
NFATC1-007 - ENSP00000389377 [100%]
NFATC1-009 - ENSP00000327850 [100%]
NFATC1-010 - ENSP00000466489 [100%]
NFATC1-011 - ENSP00000467181 [100%]

Other gene match

ANTIGEN VIEW

NFATC1-001	NFATC1-003	NFATC1-004	NFATC1-005	NFATC1-006	NFATC1-007	NFATC1-009	NFATC1-010	NFATC1-011

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.