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AOC3

ANTIBODY INFORMATION
 
 

Antibody HPA000980

  

Antibody CAB025797

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   R&D Systems
  

Product name

 HPA000980   MAB3957  

Host species

 Rabbit   Mouse  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

    

Released in version

 2   6  

References

 3    

Proper citation

 Atlas Antibodies Cat#HPA000980, RRID:AB_1078170    

VALIDATION SUMMARY
 
 

Antibody HPA000980

  

Antibody CAB025797

  
Immunocytochemistry

Uncertain    
Immunohistochemistry

Supported   Supported  
Western blot

Supported   Supported  
Protein array

Approved    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA000980

  

Antibody CAB025797

  

Standard validation

 Uncertain    

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to cytosol & the Golgi apparatus.    

Antibody dilution

 1:25    

Literature conformity

 The subcellular location is not consistent with literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA000980

  

Antibody CAB025797

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human rectum shows distinct positivity in endothelial cells and smooth muscle.   Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 17 cell types		   RNA: detected in 36 tissues
Protein: detected in 22 cell types		  

Retrieval

 None   HIER pH6  

Antibody dilution

 1:50   1:2250  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA000980

  

Antibody CAB025797

  

Standard validation

 Supported

Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401227)		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 84.6, 70.7   84.6, 70.7, 24.5, 17.3, 12.9  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA000980

  

Antibody CAB025797

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:2000    

ANTIGEN INFORMATION
 
 

Antibody HPA000980

  

Antibody CAB025797

  

Antigen

 Recombinant protein fragment   Recombinant protein  

Length (aa)

 114    

Antigen sequence

 
DIDQMIFNRELPQASGLLHHCCFYKHRGRNLVTMTTAPRGLQSGDRATWF
GLYYNISGAGFFLHHVGLELLVNHKALDPARWTIQKVFYQGRYYDSLAQL
EAQFEAGLVNVVLI
  

											 
																				

																		

										
											
Matching transcripts

												
																					AOC3-001 - ENSP00000312326 [100%] 
AOC3-201 - ENSP00000484312 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
AOC3-001
AOC3-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA000980

												
										 
																				
											
											
											
Antibody CAB025797

												
										 
																		

																	

										
											
1.

												
																						From gene expression analysis to tissue microarrays: a rational approach to identify therapeutic and diagnostic targets in lymphoid malignancies

	Ek S et al

	Mol Cell Proteomics 2006;5(6):1072-81

			
											 
																						
											 
																				

																		

										
											
2.

												
																						Vascular adhesion protein-1 promotes liver inflammation and drives hepatic fibrosis

	Weston CJ et al

	J Clin Invest 2015;125(2):501-20

			Application: IHC				
											 
																						
											 
																				

																		

										
											
3.

												
																						Evaluation of serum and tissue levels of VAP-1 in colorectal cancer

	Ward ST et al

	BMC Cancer 2016;16:154

			Application: IHC				
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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