PDHA1 - Antibodies - The Human Protein Atlas

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PDHA1

TISSUE

premium

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA047487

Antibody HPA047864

Antibody HPA063053

Provider

Atlas Antibodies
Sigma-Aldrich

Atlas Antibodies
Sigma-Aldrich

Atlas Antibodies
Sigma-Aldrich

Product name

HPA047487

HPA047864

HPA063053

Host species

Rabbit

Rabbit

Rabbit

Clonality

pAb

pAb

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Affinity purified using the PrEST-antigen as affinity ligand

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

PDHA2 - ENSG00000163114 [100%]

PDHA2 - ENSG00000163114 [100%]

PDHA2 - ENSG00000163114 [100%]

Released in version

12

12

13

References

Proper citation

Atlas Antibodies Cat#HPA047487, RRID:AB_2680066

Atlas Antibodies Cat#HPA047864, RRID:AB_2680190

Atlas Antibodies Cat#HPA063053, RRID:AB_2684931

VALIDATION SUMMARY

Antibody HPA047487

Antibody HPA047864

Antibody HPA063053

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA047487

Antibody HPA047864

Antibody HPA063053

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line HeLa shows localization to mitochondria.

Antibody dilution

1:34

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA047487

Antibody HPA047864

Antibody HPA063053

Standard validation

Approved

Approved

Approved

Figure description

Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in subset of cells in seminiferus ducts.

Immunohistochemical staining of human testis shows distinct cytoplasmic positivity in Leydig cells.

Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts, Leydig cells were strongly stained.

Expression

RNA: detected in 37 tissues
Protein: detected in 20 cell types

RNA: detected in 37 tissues
Protein: detected in 44 cell types

RNA: detected in 37 tissues
Protein: detected in 77 cell types

Retrieval

HIER pH6

HIER pH6

HIER pH6

Antibody dilution

1:50

1:75

1:900

Literature conformity

Not consistent with gene/protein characterization data.

Consistent with extensive gene/protein characterization data.

Consistent with extensive gene/protein characterization data.

RNA consistency

Not consistent with RNA expression data.

Mainly consistent with RNA expression data.

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA047487

Antibody HPA047864

Antibody HPA063053

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

47.6, 44.1, 43.3, 42.9, 40.2, 22.8, 22.3, 19.8

47.6, 44.1, 43.3, 42.9, 40.2, 22.8, 22.3, 19.8, 13.8

47.6, 44.1, 43.3, 42.9, 40.2

Antibody dilution

1:220

1:170

1:150

PROTEIN ARRAY

Antibody HPA047487

Antibody HPA047864

Antibody HPA063053

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:500

1:1350

1:1750

ANTIGEN INFORMATION

Antibody HPA047487

Antibody HPA047864

Antibody HPA063053

Antigen

Recombinant protein fragment

Recombinant protein fragment

Recombinant protein fragment

Length (aa)

35

27

29

Antigen sequence

ILAELTGRRGGCAKGKGGSMHMYTKNFYGGNGIVG

NDATFEIKKCDLYLLEEGPPVTTVLTR

YCRSGKGPILMELQTYRYHGHSMSDPGVS

Matching transcripts

PDHA1-001 - ENSP00000369133 [94%]
PDHA1-004 - ENSP00000394382 [94%]
PDHA1-008 - ENSP00000348062 [94%]
PDHA1-201 - ENSP00000369134 [94%]
PDHA1-202 - ENSP00000440761 [94%]
PDHA1-203 - ENSP00000438550 [94%]
PDHA1-009 - ENSP00000406473 [91%]

PDHA1-001 - ENSP00000369133 [93%]
PDHA1-004 - ENSP00000394382 [93%]
PDHA1-008 - ENSP00000348062 [93%]
PDHA1-009 - ENSP00000406473 [93%]
PDHA1-010 - ENSP00000404616 [93%]
PDHA1-201 - ENSP00000369134 [93%]
PDHA1-202 - ENSP00000440761 [93%]
PDHA1-203 - ENSP00000438550 [93%]

PDHA1-004 - ENSP00000394382 [100%]
PDHA1-201 - ENSP00000369134 [100%]
PDHA1-202 - ENSP00000440761 [100%]
PDHA1-203 - ENSP00000438550 [100%]

Other gene match

PDHA2 - ENSG00000163114 [100%]

PDHA2 - ENSG00000163114 [100%]

PDHA2 - ENSG00000163114 [100%]

ANTIGEN VIEW

PDHA1-001	PDHA1-004	PDHA1-008	PDHA1-009	PDHA1-010	PDHA1-201	PDHA1-202	PDHA1-203

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.