RPA1 - Antibodies - The Human Protein Atlas

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RPA1

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA006914

Antibody HPA046497

Antibody CAB004563

Provider

Atlas Antibodies
Sigma-Aldrich

Santa Cruz Biotechnology

Product name

HPA006914

HPA046497

sc-28304

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Protein A/G

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA006914, RRID:AB_1856410

Atlas Antibodies Cat#HPA046497, RRID:AB_2679675

Santa Cruz Biotechnology Cat#sc-28304, RRID:AB_628224

VALIDATION SUMMARY

Antibody HPA006914

Antibody HPA046497

Antibody CAB004563

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA006914

Antibody HPA046497

Antibody CAB004563

Formal validation: Tagged protein

Validated

Antibody staining overlaps with GFP tagged protein

Figure description

Immunofluorescent staining of transgenic HeLa cells show antibody staining in nucleoplasm and GFP expression in nucleoplasm.

Antibody dilution

1:128

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.

Antibody dilution

1:128

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA006914

Antibody HPA046497

Antibody CAB004563

Standard validation

Supported

Figure description

Immunohistochemical staining of human placenta shows strong nuclear positivity in trophoblastic cells.

Immunohistochemical staining of human placenta shows strong nuclear and cytoplasmic positivity in trophoblastic cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 75 cell types

RNA: detected in 37 tissues
Protein: detected in 51 cell types

Retrieval

HIER pH6

Antibody dilution

1:25

1:15

Literature conformity

Partly consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA006914

Antibody HPA046497

Antibody CAB004563

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

68.1

68.1, 35.2, 16.3, 13.4

Antibody dilution

1:250

1:640

1:500

PROTEIN ARRAY

Antibody HPA006914

Antibody HPA046497

Antibody CAB004563

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:5100

ANTIGEN INFORMATION

Antibody HPA006914

Antibody HPA046497

Antibody CAB004563

Antigen

Recombinant protein fragment

Recombinant protein

Length (aa)

136

129

Antigen sequence

SLSHTSGGTQSKVVPIASLTPYQSKWTICARVTNKSQIRTWSNSRGEGKL
FSLELVDESGEIRATAFNEQVDKFFPLIEVNKVYYFSKGTLKIANKQFTA
VKNDYEMTFNNETSVMPCEDDHHLPTVQFDFTGIDD

PVLAIKGARVSDFGGRSLSVLSSSTIIANPDIPEAYKLRGWFDAEGQALD
GVSISDLKSGGVGGSNTNWKTLYEVKSENLGQGDKPDYFSSVATVVYLRK
ENCMYQACPTQDCNKKVIDQQNGLYRCEK

Matching transcripts

RPA1-001 - ENSP00000254719 [100%]

Other gene match

ANTIGEN VIEW

RPA1-001

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.