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Field

Term
Gene name

Class

Subclass

Chromosome

External id

In atlas

Reliability

Reliability

Reliability

Validation

Method

Validation

Method

Validation

Method

Validation

Location

Searches

Tissue

Cell type

Expression

Tissue

Specificity

Cell line

Specificity

Cancer

Specificity

Tissue

Detectable

Cell line

Detectable

Cancer

Detectable

Cancer

Prognosis

Category

Score

Score

Score

Antibodies

Column

 
 
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RPA1
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    TISSUE

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    CELL

  • PATHOLOGY

PATHOLOGY ATLAS

GENE/PROTEIN

Antibody validation

Dictionary

Level of antibody staining/expression





High
Medium
Low
Not detected

GENERAL INFORMATION

Gene name

RPA1

Gene description

Replication protein A1, 70kDa

Protein class

Plasma proteins
Predicted intracellular proteins

Predicted localization

Intracellular

Number of transcripts

4
SHOW MORE

HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):Expressed in all
HPA (cell line):Expressed in all
HPA (normal tissue):Expressed in all

Protein evidence

Evidence at protein level

Protein expression
normal tissue

General nuclear expression.

ANTIBODY IHC RELIABILITY

Data reliability
description

Antibody staining mainly consistent with RNA expression data.

Reliability score

Supported based on 2 antibodies.
HPA006914 , CAB004563
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PROGNOSTIC SUMMARY

Prognostic marker in liver cancer (unfavourable).
Liver cancer p<0.001
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RNA EXPRESSION OVERVIEW

TCGA dataset
RNA cancer category: Expressed in all

PROTEIN EXPRESSION

Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer
Liver cancer

STAINING SUMMARY

HPA006914
CAB004563

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Cancer cells showed moderate to strong nuclear staining. Additional cytoplasmic positivity in a few cases. Liver cancers showed generally strong immunoreactivity in cholangiocarcinomas. Hepatocellular cancers stained weakly.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

A majority of cancers showed moderate to strong nuclear and cytoplasmic positivity. Prostate cancer were mainly negative.

GENE INFORMATION

Gene name

RPA1 (HGNC Symbol)

Synonyms

HSSB, REPA1, RF-A, RP-A, RPA70

Description

Replication protein A1, 70kDa (HGNC Symbol)

Entrez gene summary

Chromosome

17

Cytoband

p13.3

Chromosome location (bp)

1829702 - 1900082

Protein evidence

Evidence at protein level (all genes)

Ensembl

ENSG00000132383 (version 83.38)

Entrez gene

6117

UniProt

P27694 (UniProt - Evidence at protein level)

neXtProt

NX_P27694

Antibodypedia

RPA1 antibodies

PROTEIN BROWSER

RPA1-001
RPA1-002
RPA1-003
RPA1-006

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)

RPA1-001
ENSP00000254719
ENST00000254719
P27694 [Direct mapping]
Replication protein A 70 kDa DNA-binding subunit Replication protein A 70 kDa DNA-binding subunit, N-terminally processed
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Predicted intracellular proteins
Plasma proteins
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000082 [G1/S transition of mitotic cell cycle]
GO:0000278 [mitotic cell cycle]
GO:0000722 [telomere maintenance via recombination]
GO:0000723 [telomere maintenance]
GO:0000724 [double-strand break repair via homologous recombination]
GO:0000784 [nuclear chromosome, telomeric region]
GO:0000793 [condensed chromosome]
GO:0000794 [condensed nuclear chromosome]
GO:0000800 [lateral element]
GO:0001673 [male germ cell nucleus]
GO:0001701 [in utero embryonic development]
GO:0003676 [nucleic acid binding]
GO:0003677 [DNA binding]
GO:0003682 [chromatin binding]
GO:0003684 [damaged DNA binding]
GO:0003697 [single-stranded DNA binding]
GO:0005515 [protein binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005662 [DNA replication factor A complex]
GO:0006260 [DNA replication]
GO:0006261 [DNA-dependent DNA replication]
GO:0006271 [DNA strand elongation involved in DNA replication]
GO:0006281 [DNA repair]
GO:0006283 [transcription-coupled nucleotide-excision repair]
GO:0006284 [base-excision repair]
GO:0006289 [nucleotide-excision repair]
GO:0006297 [nucleotide-excision repair, DNA gap filling]
GO:0006298 [mismatch repair]
GO:0006302 [double-strand break repair]
GO:0006310 [DNA recombination]
GO:0007126 [meiotic nuclear division]
GO:0008284 [positive regulation of cell proliferation]
GO:0016605 [PML body]
GO:0016925 [protein sumoylation]
GO:0019985 [translesion synthesis]
GO:0030097 [hemopoiesis]
GO:0032201 [telomere maintenance via semi-conservative replication]
GO:0033683 [nucleotide-excision repair, DNA incision]
GO:0034605 [cellular response to heat]
GO:0036297 [interstrand cross-link repair]
GO:0042276 [error-prone translesion synthesis]
GO:0042769 [DNA damage response, detection of DNA damage]
GO:0043687 [post-translational protein modification]
GO:0044267 [cellular protein metabolic process]
GO:0046872 [metal ion binding]
GO:0048873 [homeostasis of number of cells within a tissue]
GO:0051276 [chromosome organization]
GO:0070911 [global genome nucleotide-excision repair]
GO:0070987 [error-free translesion synthesis]
GO:1900034 [regulation of cellular response to heat]
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616 aa
68.1 kDa
No 0
RPA1-002
ENSP00000461733
ENST00000571058
I3L524 [Direct mapping]
Replication protein A 70 kDa DNA-binding subunit
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0003677 [DNA binding]
GO:0005634 [nucleus]
GO:0006260 [DNA replication]
Show all
124 aa
13.4 kDa
No 0
RPA1-003
ENSP00000459788
ENST00000570451
I3L2M5 [Direct mapping]
Replication protein A 70 kDa DNA-binding subunit
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0003677 [DNA binding]
GO:0005634 [nucleus]
GO:0006260 [DNA replication]
Show all
153 aa
16.3 kDa
No 0
RPA1-006
ENSP00000461466
ENST00000574049
I3L4R8 [Direct mapping]
Replication protein A 70 kDa DNA-binding subunit
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
313 aa
35.2 kDa
No 0

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