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UBE2G1

ANTIBODY INFORMATION
 
 

Antibody HPA045681

  

Antibody HPA050551

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA045681   HPA050551  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 10   10  

References

    

Proper citation

 Atlas Antibodies Cat#HPA045681, RRID:AB_2679417   Atlas Antibodies Cat#HPA050551, RRID:AB_2681171  

VALIDATION SUMMARY
 
 

Antibody HPA045681

  

Antibody HPA050551

  
Immunocytochemistry

  Approved  
Immunohistochemistry

Approved   Approved  
Western blot

Supported   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA045681

  

Antibody HPA050551

  

Standard validation

   Approved  

   

Figure description

   Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm & cytosol.  

Antibody dilution

   1:10  

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA045681

  

Antibody HPA050551

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 42 cell types		   RNA: detected in 37 tissues
Protein: detected in 76 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:50   1:20  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA045681

  

Antibody HPA050551

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 19.5   19.5, 11.4, 9  

Antibody dilution

 1:250   1:100  

PROTEIN ARRAY
 
 

Antibody HPA045681

  

Antibody HPA050551

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:800  

ANTIGEN INFORMATION
 
 

Antibody HPA045681

  

Antibody HPA050551

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 51   49  

Antigen sequence

 
MTELQSALLLRRQLAELNKNPVEGFSAGLIDDNDLYRWEVLIIGPPDTLY
E
  
RWLPIHTVETIMISVISMLADPNGDSPANVDAAKEWREDRNGEFKRKVA
 

Matching transcripts

 UBE2G1-001 - ENSP00000380178 [100%]
  UBE2G1-001 - ENSP00000380178 [100%]
UBE2G1-002 - ENSP00000459691 [100%]
UBE2G1-004 - ENSP00000467502 [82%]
 

Other gene match

    
ANTIGEN VIEW
UBE2G1-001
UBE2G1-002
UBE2G1-004
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.