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Antibody staining overlaps with GFP tagged protein
Figure description
Immunofluorescent staining of transgenic HeLa cells show antibody staining in cytoplasm, nucleoli (fibrillar center) & nucleoplasm and GFP expression in cytoplasm & nucleoplasm.
Antibody dilution
1:45
Standard validation
Supported
Figure description
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nucleoli fibrillar center.
Antibody dilution
1:45
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA044241
Standard validation
Supported
Figure description
Immunohistochemical staining of human duodenum shows strong cytoplasmic and membranous positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 69 cell types
Retrieval
HIER pH6
Antibody dilution
1:25
Literature conformity
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA044241
Standard validation
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
78.5, 73.2
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA044241
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
