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Field

Term
Gene name

Class

Subclass

Chromosome

External id

In atlas

Reliability

Reliability

Reliability

Validation

Method

Validation

Method

Validation

Method

Validation

Location

Searches

Tissue

Cell type

Expression

Tissue

Specificity

Cell line

Specificity

Cancer

Specificity

Tissue

Detectable

Cell line

Detectable

Cancer

Detectable

Cancer

Prognosis

Category

Score

Score

Score

Antibodies

Column

 
 
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VPS4A
  • TISSUE

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    CELL

  • PATHOLOGY

PATHOLOGY ATLAS

GENE/PROTEIN

Antibody validation

Dictionary

Level of antibody staining/expression





High
Medium
Low
Not detected

GENERAL INFORMATION

Gene name

VPS4A

Gene description

Vacuolar protein sorting 4 homolog A (S. cerevisiae)

Protein class

Enzymes
Predicted intracellular proteins

Predicted localization

Intracellular

Number of transcripts

1
SHOW MORE

HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):Expressed in all
HPA (cell line):Expressed in all
HPA (normal tissue):Expressed in all

Protein evidence

Evidence at protein level

Protein expression
normal tissue

Ubiquitous cytoplasmic expression.

ANTIBODY IHC RELIABILITY

Data reliability
description

Antibody staining mainly not consistent with RNA expression data.

Reliability score

Approved based on 2 antibodies.
CAB018751 , CAB034411
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PROGNOSTIC SUMMARY

Prognostic marker in renal cancer (favourable).
Renal cancer p<0.001
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RNA EXPRESSION OVERVIEW

TCGA dataset
RNA cancer category: Expressed in all

PROTEIN EXPRESSION

Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer
Liver cancer

STAINING SUMMARY

CAB018751
CAB034411

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Cancer cells showed weak to moderate cytoplasmic immunoreactivity. Most breast cancers and several cases of lymphomas, skin and lung cancers were negative.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Cancer tissues displayed moderate to strong cytoplasmic positivity with a granular pattern. Additional membranous positivity in few cases. Several cases of liver cancers were negative.

GENE INFORMATION

Gene name

VPS4A (HGNC Symbol)

Synonyms

FLJ22197, SKD1, SKD1A, SKD2, VPS4, VPS4-1

Description

Vacuolar protein sorting 4 homolog A (S. cerevisiae) (HGNC Symbol)

Entrez gene summary

The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. The mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be really an yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 16; the gene for the other resides on chromosome 18. [provided by RefSeq, Jul 2008]

Chromosome

16

Cytoband

q22.1

Chromosome location (bp)

69311356 - 69326939

Protein evidence

Evidence at protein level (all genes)

Ensembl

ENSG00000132612 (version 83.38)

Entrez gene

27183

UniProt

Q9UN37 (UniProt - Evidence at protein level)

neXtProt

NX_Q9UN37

Antibodypedia

VPS4A antibodies

PROTEIN BROWSER

VPS4A-002

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)

VPS4A-002
ENSP00000254950
ENST00000254950
Q9UN37 [Direct mapping]
Vacuolar protein sorting-associated protein 4A
A0A024R705 [Target identity:100%; Query identity:100%]
Vacuolar protein sorting 4A (Yeast), isoform CRA_c
Show all
Enzymes
   ENZYME proteins
   Hydrolases
Predicted intracellular proteins
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000815 [ESCRT III complex]
GO:0000920 [cell separation after cytokinesis]
GO:0000922 [spindle pole]
GO:0005515 [protein binding]
GO:0005524 [ATP binding]
GO:0005634 [nucleus]
GO:0005737 [cytoplasm]
GO:0005764 [lysosome]
GO:0005768 [endosome]
GO:0005769 [early endosome]
GO:0005770 [late endosome]
GO:0005774 [vacuolar membrane]
GO:0005813 [centrosome]
GO:0005829 [cytosol]
GO:0005886 [plasma membrane]
GO:0006900 [membrane budding]
GO:0006997 [nucleus organization]
GO:0007033 [vacuole organization]
GO:0007080 [mitotic metaphase plate congression]
GO:0008022 [protein C-terminus binding]
GO:0009838 [abscission]
GO:0015031 [protein transport]
GO:0016032 [viral process]
GO:0016192 [vesicle-mediated transport]
GO:0016197 [endosomal transport]
GO:0016887 [ATPase activity]
GO:0019058 [viral life cycle]
GO:0019076 [viral release from host cell]
GO:0019904 [protein domain specific binding]
GO:0030496 [midbody]
GO:0031902 [late endosome membrane]
GO:0032367 [intracellular cholesterol transport]
GO:0032466 [negative regulation of cytokinesis]
GO:0032880 [regulation of protein localization]
GO:0034058 [endosomal vesicle fusion]
GO:0039702 [viral budding via host ESCRT complex]
GO:0042623 [ATPase activity, coupled]
GO:0043162 [ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway]
GO:0048471 [perinuclear region of cytoplasm]
GO:0051301 [cell division]
GO:0061024 [membrane organization]
GO:0070062 [extracellular exosome]
GO:0072319 [vesicle uncoating]
GO:0090543 [Flemming body]
GO:0090611 [ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway]
GO:1902188 [positive regulation of viral release from host cell]
GO:1903076 [regulation of protein localization to plasma membrane]
GO:1903543 [positive regulation of exosomal secretion]
GO:1903774 [positive regulation of viral budding via host ESCRT complex]
GO:1903902 [positive regulation of viral life cycle]
Show all
437 aa
48.9 kDa
No 0

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