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ANTIBODY INFORMATION
 
 

Antibody HPA006286

  

Antibody HPA007007

  

Antibody HPA026111

  

Antibody CAB005889

  

Antibody CAB058692

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Abcam plc
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA006286   HPA007007   HPA026111   ab5968   AMAb90556  

Host species

 Rabbit   Rabbit   Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   pAb   msAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity   Protein A/G  

Other gene match

          

Released in version

 14   4   5   3   11  

References

   2        

Proper citation

 Atlas Antibodies Cat#HPA006286, RRID:AB_2667550   Atlas Antibodies Cat#HPA007007, RRID:AB_1854381   Atlas Antibodies Cat#HPA026111, RRID:AB_1854382   Abcam Cat#ab5968, RRID:AB_2251301   Atlas Antibodies Cat#AMAb90556, RRID:AB_2665584  

VALIDATION SUMMARY
 
 

Antibody HPA006286

  

Antibody HPA007007

  

Antibody HPA026111

  

Antibody CAB005889

  

Antibody CAB058692

  
Immunocytochemistry


Validated

Validated
    

Validated

Validated
   Supported    
Immunohistochemistry

 

Validated

Validated
  

Validated

Validated
  

Validated
  

Validated
  
Western blot

Uncertain  

Validated
   Uncertain   Uncertain   Uncertain  
Protein array

Approved   Supported   Supported      

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA006286

  

Antibody HPA007007

  

Antibody HPA026111

  

Antibody CAB005889

  

Antibody CAB058692

  

Formal validation: Tagged protein
Validated

Antibody staining overlaps with GFP tagged protein		    
Validated

Antibody staining overlaps with GFP tagged protein		      

         

Figure description

 Immunofluorescent staining of transgenic HeLa cells show antibody staining in intermediate filaments and GFP expression in intermediate filaments.     Immunofluorescent staining of transgenic HeLa cells show antibody staining in intermediate filaments and GFP expression in intermediate filaments.      

Antibody dilution

 1:60     1:200      
 

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA026111.		    
Validated

Antibody staining overlaps with antibody HPA006286.		      
 

Standard validation

 Supported     Supported   Supported    

         

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to intermediate filaments.     Immunofluorescent staining of human cell line U-251 MG shows localization to intermediate filaments.   Immunofluorescent staining of human cell line U-2 OS shows localization to intermediate filaments.    

Antibody dilution

 1:60     1:200   1:200    

Literature conformity

 The subcellular location is supported by literature.     The subcellular location is supported by literature.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA006286

  

Antibody HPA007007

  

Antibody HPA026111

  

Antibody CAB005889

  

Antibody CAB058692

  

Formal validation: Orthogonal

  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 
 
 
 
 

Figure description

   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Formal validation: Independent

  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		      

 
 
     

Figure description

   Distribution of protein expression (antibody staining).
Pearson correlation with HPA026111 across 44 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA007007 across 44 cell lines.		      
 

Standard validation

   Supported   Supported   Supported   Supported  

         

Figure description

   Immunohistochemical staining of human kidney shows distinct positivity in cells in glomeruli.   Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in glomeruli and endothlial cells.   Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in glomeruli.   Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in glomeruli.  

Expression

   RNA: detected in 35 tissues
Protein: detected in 14 cell types		   RNA: detected in 35 tissues
Protein: detected in 13 cell types		   RNA: detected in 35 tissues
Protein: detected in 58 cell types		   RNA: detected in 35 tissues
Protein: detected in 8 cell types		  

Retrieval

   HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

   1:250   1:3000   1:1000   1:3500  

Literature conformity

   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

   Consistent with RNA expression data.   Consistent with RNA expression data.   Mainly consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA006286

  

Antibody HPA007007

  

Antibody HPA026111

  

Antibody CAB005889

  

Antibody CAB058692

  

Formal validation: Genetic

  
Validated

Downregulation visible in both siRNA lanes		        

         

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		        

Target mass (kDa)

   177.4        

Loading control

   Total protein image        

Antibody dilution

   1:81        
 

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.		  

         

Figure description

         Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 177.4   177.4   177.4   177.4   177.4  

Antibody dilution

 1:250   1:250   1:250   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA006286

  

Antibody HPA007007

  

Antibody HPA026111

  

Antibody CAB005889

  

Antibody CAB058692

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		      

         

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:3000   1:3000   1:3000      

ANTIGEN INFORMATION
 
 

Antibody HPA006286

  

Antibody HPA007007

  

Antibody HPA026111

  

Antibody CAB005889

  

Antibody CAB058692

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Synthetic peptide   Recombinant protein  

Length (aa)

 119   119   144      

Antigen sequence

 
DPEGQSQQVGAPGLQAPQGLPEAIEPLVEDDVAPGGDQASPEVMLGSEPA
MGESAAGAEPGPGQGVGGLGDPGHLTREEVMEPPLEEESLEAKRVQGLEG
PRKDLEEAGGLGTEFSELP
  
DPEGQSQQVGAPGLQAPQGLPEAIEPLVEDDVAPGGDQASPEVMLGSEPA
MGESAAGAEPGPGQGVGGLGDPGHLTREEVMEPPLEEESLEAKRVQGLEG
PRKDLEEAGGLGTEFSELP
  
EPLRSLEDENKEAFRSLEKENQEPLKTLEEEDQSIVRPLETENHKSLRSL
EEQDQETLRTLEKETQQRRRSLGEQDQMTLRPPEKVDLEPLKSLDQEIAR
PLENENQEFLKSLKEESVEAVKSLETEILESLKSAGQENLETLK
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					NES-001 - ENSP00000357206 [100%] 

											 
																						NES-001 - ENSP00000357206 [100%] 

											 
																						NES-001 - ENSP00000357206 [100%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
NES-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA006286

												
										 
																				
											
											
											
Antibody HPA007007

												
										 
																				
											
											
											
Antibody HPA026111

												
										 
																				
											
											
											
Antibody CAB005889

												
										 
																				
											
											
											
Antibody CAB058692

												
										 
																		

																	

										
											
1.

												
																					
											 
																							Spatiotemporal expression pattern of neuroepithelial stem cell marker nestin suggests a role in dermal homeostasis, neovasculogenesis, and tumor stroma development: a study on embryonic and adult human skin

	Sellheyer K et al

	J Am Acad Dermatol 2010;63(1):93-113

			
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
2.

												
																					
											 
																							Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy

	Stadler C et al

	J Proteomics 2012;75(7):2236-51

			
											 
																						
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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