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Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in a subset of proximal tubules.
Immunohistochemical staining of human kidney shows moderate cytoplasmic and nuclear positivity in proximal renal tubules.
Expression
RNA: detected in 20 tissues Protein: detected in 26 cell types
RNA: detected in 20 tissues Protein: detected in 13 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:150
1:1500
Literature conformity
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA039219
Antibody HPA048187
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY408962)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY408962)
Target mass (kDa)
35.2, 22
35.2
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA039219
Antibody HPA048187
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
