INADL - Antibodies - The Human Protein Atlas

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INADL

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA066352

Antibody HPA066960

Antibody HPA069079

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA066352

HPA066960

HPA069079

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA066352, RRID:AB_2685663

Atlas Antibodies Cat#HPA066960, RRID:AB_2685755

Atlas Antibodies Cat#HPA069079, RRID:AB_2686079

VALIDATION SUMMARY

Antibody HPA066352

Antibody HPA066960

Antibody HPA069079

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA066352

Antibody HPA066960

Antibody HPA069079

Formal validation: Independent

Validated

Antibody staining overlaps with antibody HPA069079.

Validated

Antibody staining overlaps with antibody HPA066960.

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to microtubule organizing center & cell junctions.

Immunofluorescent staining of human cell line A-431 shows localization to cytosol, microtubule organizing center & cell junctions.

Antibody dilution

1:143

1:76

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA066352

Antibody HPA066960

Antibody HPA069079

Standard validation

Approved

Figure description

Immunohistochemical staining of human colon shows strong membranous positivity in glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 59 cell types

Retrieval

HIER pH6

Antibody dilution

1:400

Literature conformity

Partly consistent with extensive gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

WESTERN BLOT

Antibody HPA066352

Antibody HPA066960

Antibody HPA069079

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Figure description

Target mass (kDa)

196.4, 63.4

196.4, 130.1, 130

Antibody dilution

1:110

1:480

1:250

PROTEIN ARRAY

Antibody HPA066352

Antibody HPA066960

Antibody HPA069079

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:1200

1:5700

1:3050

ANTIGEN INFORMATION

Antibody HPA066352

Antibody HPA066960

Antibody HPA069079

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

ATILKCAQGLVQLEIGRLRAGSWTSARTTSQNSQGSQQSAHSSCHPSFAP
VITGLQNLVGTKRVSDPSQKNSGTDMEPRTVEI

QLYGKSRREAVSFLKEVPPPFTLVCCRRLFDDEASVDEPRRTETSLPETE
VDHNMDVNTEEDDDGELALWSPEVKIVELVKDCKG

NDNIQALEKLEKVPDSPENELKSRWENLLGPDYEVMVATLDTQIADDAEL
QKYSKLLPIHTLRLGVEVDSFDGHHYISSI

Matching transcripts

INADL-001 - ENSP00000360200 [100%]
INADL-008 - ENSP00000489159 [100%]

INADL-001 - ENSP00000360200 [100%]
INADL-201 - ENSP00000326199 [100%]
INADL-202 - ENSP00000479041 [100%]

Other gene match

ANTIGEN VIEW

INADL-001	INADL-008	INADL-201	INADL-202

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.