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Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & intermediate filaments.
Antibody dilution
1:192
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA044140
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts and leydig cells).
Expression
RNA: detected in 36 tissues Protein: detected in 77 cell types
Retrieval
HIER pH6
Antibody dilution
1:500
Literature conformity
Consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA044140
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY404577)
Target mass (kDa)
105.2, 100
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA044140
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
