MORC4 - Antibodies - The Human Protein Atlas

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MORC4

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA000395

Antibody HPA015547

Antibody HPA050250

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA000395

HPA015547

HPA050250

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA000395, RRID:AB_1079405

Atlas Antibodies Cat#HPA015547, RRID:AB_2669216

Atlas Antibodies Cat#HPA050250, RRID:AB_2681063

VALIDATION SUMMARY

Antibody HPA000395

Antibody HPA015547

Antibody HPA050250

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA000395

Antibody HPA015547

Antibody HPA050250

Formal validation: Independent

Validated

Antibody staining overlaps with antibody HPA050250.

Validated

Antibody staining overlaps with antibody HPA000395.

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm.

Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.

Antibody dilution

1:50

1:7

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA000395

Antibody HPA015547

Antibody HPA050250

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.

Formal validation: Independent

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Pearson correlation with HPA015547 across 46 cell lines.

Distribution of protein expression (antibody staining).
Pearson correlation with HPA000395 across 46 cell lines.

Standard validation

Supported

Figure description

Immunohistochemical staining of human testis shows moderate nuclear positivity in a subset of cells in seminiferous ducts.

Immunohistochemical staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.

Expression

RNA: detected in 37 tissues
Protein: detected in 4 cell types

RNA: detected in 37 tissues
Protein: detected in 18 cell types

Retrieval

HIER pH6

Antibody dilution

1:35

1:75

Literature conformity

Consistent with gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA000395

Antibody HPA015547

Antibody HPA050250

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

106.3, 101.9

Antibody dilution

1:250

1:80

PROTEIN ARRAY

Antibody HPA000395

Antibody HPA015547

Antibody HPA050250

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:1000

1:3000

1:500

ANTIGEN INFORMATION

Antibody HPA000395

Antibody HPA015547

Antibody HPA050250

Antigen

Recombinant protein fragment

Length (aa)

125

121

Antigen sequence

PRLVAEESNRGSTTINKEEVNKGPFVAVVGVAKGVRDSGAPIQLIPFNRE
ELAERRKAVESWNPVPYSVASAAIPAAAIGEKARGYEESEGHNTPKLKNQ
RELEELKRTTEKLERVLAERNLFQQ

SPSLQLKPLDSSVLQFSSKYKWILGEEPVEKRRRLQNEMTTPSLDYSMPA
PYRRVEAPVAYPEGENSHDKSSSERSTPPYLFPEYPEASKNTGQNREVSI
LYPGAKDQRQGSLLPEELEDQ

SPSLQLKPLDSSVLQFSSKYKWILGEEPVEKRRRLQNEMTTPSLDYSMPA
PYRRVEAPVAYPEGENSHDKSSSERSTPPYLFPEYPEASKNTGQNREVSI
LYPGAKDQRQGSLLPEELEDQ

Matching transcripts

MORC4-001 - ENSP00000347821 [100%]
MORC4-003 - ENSP00000255495 [100%]

Other gene match

ANTIGEN VIEW

MORC4-001	MORC4-003

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.